The chemistry program enables the use of chemical probes to understand biologic pathways and phenotype cellular states in health and disease. These probes may be directed at validated disease targets, or applied in a "forward genetic" approach that is systematic and less dependent on a priori assumptions. Platform activities encompass three interrelated efforts: 1) Synthetic chemistry, including the creation of novel small molecules, diversity oriented synthesis (DOS) and other libraries; 2) High-throughput screening and analysis, utilizing a variety of biochemical, metabolic, and high-content imaging measurements and 3) a Chemistry Core for the production and quality control of imaging probes that are not commercially available.
Ko J, Lucas K, Kohler RH, Halabi EA, Wilkovitsch M, Carlson JCT, Weissleder R In Vivo Click Chemistry Enables Multiplexed Intravital Microscopy. Adv Sci (Weinh). 2022;:e2200064 - PMID: 35750648 - DOI: 10.1002/advs.202200064
Ko J, Wilkovitsch M, Oh J, Kohler RH, Bolli E, Pittet MJ, Vinegoni C, Sykes DB, Mikula H, Weissleder R, Carlson JCT Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes. Nat Biotechnol. 2022;:ePub - PMID: 35654978 - DOI: 10.1038/s41587-022-01339-6
Payne NC, Maksoud S, Tannous BA, Mazitschek R A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem Biol. 2022;:ePub - PMID: 35649410 - DOI: 10.1016/j.chembiol.2022.05.003
Quintana JM, Arboleda D, Hu H, Scott E, Luthria G, Pai S, Parangi S, Weissleder R, Miller MA Radiation Cleaved Drug-Conjugate Linkers Enable Local Payload Release. Bioconjug Chem. 2022;33(8):1474-1484 - PMID: 35833631 - DOI: 10.1021/acs.bioconjchem.2c00174
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Kritika Singh (Mazitschek Lab), a bioengineering and chemistry student at Northeastern who has dedicated herself to tackling global health issues, has been named a 2020 Rhodes Scholar, considered the most prestigious academic honor for U.S. college students. Congratulations, Kritika!
Novel imaging probe platform developed at CSB enables imaging - "BODIPY–Tetrazine Derivatives as Superbright Bioorthogonal Turn-on Probes"