The chemical biology platform enables the use of chemical probes to understand biologic pathways and phenotype cellular states in health and disease. These probes may be directed at validated disease targets, or applied in a "forward genetic" approach that is systematic and less dependent on a priori assumptions. Platform activities encompass three interrelated efforts: 1) Synthetic chemistry, including the creation of novel small molecules, diversity oriented synthesis (DOS) and other libraries; 2) High-throughput screening and analysis, utilizing a variety of biochemical, metabolic, and high-content imaging measurements and 3) a Chemistry Core for the production and quality control of imaging probes that are not commercially available.
Wang Y, Cao T, Ko J, Shen Y, Zong W, Sheng K, Cao W, Sun S, Cai L, Zhou YL, Zhang XX, Zong C, Weissleder R, Weitz D Dissolvable Polyacrylamide Beads for High-Throughput Droplet DNA Barcoding. Adv Sci. 2020;7(8):1903463 - PMID: 32328429 - PMCID: PMC7175265 - DOI: 10.1002/advs.201903463
Kim Y, Sundrud MS, Zhou C, Edenius M, Zocco D, Powers K, Zhang M, Mazitschek R, Rao A, Yeo CY, Noss EH, Brenner MB, Whitman M, Keller TL Aminoacyl-tRNA synthetase inhibition activates a pathway that branches from the canonical amino acid response in mammalian cells. Proc Natl Acad Sci U S A. 2020;117(16):8900-8911 - PMID: 32253314 - PMCID: PMC7183223 - DOI: 10.1073/pnas.1913788117
Ko J, Oh J, Ahmed MS, Carlson JCT, Weissleder R Ultra-fast cycling for multiplexed cellular fluorescence imaging. Angew Chem Int Ed Engl. 2020;59(17):6839-6846 - PMID: 32004403 - DOI: 10.1002/anie.201915153
Marquard AN, Carlson JCT, Weissleder R Expanding the scope of antibody rebridging with new pyridazinedione-TCO constructs. Bioconjug Chem. 2020;31(6):1616-1623 - PMID: 32286045 - DOI: 10.1021/acs.bioconjchem.0c00155
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