The current research activity involves the development of novel optical mesoscopic molecular imaging techniques that allow to generate in-vivo three-dimensional data in optically diffusive non-transparent living organisms with a size up to a few millimeters (e.g. Drosophila Melanogaster, Zebrafish) providing both in-vivo anatomical and functional imaging. At the same time we are committed into the development of new fluorescence molecular tomography (FMT) techniques in diffusive regime for whole mouse imaging. Other research activities involve in vivo near infrared fluorescence imaging of protease activity in rabbit models of atherosclerosis and the development of novel fiber based imaging systems, and the development of combined optical and opto-acoustic multispectral tomographic imaging for in-vivo imaging applications.
Grune J, Lewis AJM, Yamazoe M, Hulsmans M, Rohde D, Xiao L, Zhang S, Ott C, Calcagno DM, Zhou Y, Timm K, Shanmuganathan M, Pulous FE, Schloss MJ, Foy BH, Capen D, Vinegoni C, Wojtkiewicz GR, Iwamoto I, Grune T, Brown D, Higgins J, Ferreira VM, Herring N, Channon KM, Neubauer S, Oxford Acute Myocardial Infarction (OxAMI) Study, Sosnovik DE, Milan DJ, Swirski FK, King KR, Aguirre AD, Ellinor PT, Nahrendorf M Neutrophils incite and macrophages avert electrical storm after myocardial infarction. Nat Cardiovasc Res. 2022;1(7):649-664 - PMID: 36034743 - PMCID: PMC9410341 - DOI: 10.1038/s44161-022-00094-w
Ko J, Wilkovitsch M, Oh J, Kohler RH, Bolli E, Pittet MJ, Vinegoni C, Sykes DB, Mikula H, Weissleder R, Carlson JCT Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes. Nat Biotechnol. 2022;40(11):1654-1662 - PMID: 35654978 - PMCID: PMC9669087 - DOI: 10.1038/s41587-022-01339-6
Pulous FE, Cruz-Hernandez JC, Yang C, Kaya Z, Paccalet A, Wojtkiewicz G, Capen D, Brown D, Wu JW, Schloss MJ, Vinegoni C, Richter D, Yamazoe M, Hulsmans M, Momin N, Grune J, Rohde D, McAlpine CS, Panizzi P, Weissleder R, Kim DE, Swirski FK, Lin CP, Moskowitz MA, Nahrendorf M Cerebrospinal fluid can exit into the skull bone marrow and instruct cranial hematopoiesis in mice with bacterial meningitis. Nat Neurosci. 2022;25(5):567-576 - PMID: 35501382 - PMCID: PMC9081225 - DOI: 10.1038/s41593-022-01060-2
Rohde D, Vandoorne K, Lee IH, Grune J, Zhang S, McAlpine CS, Schloss MJ, Nayar R, Courties G, Frodermann F, Wojtkiewicz G, Honold L, Chen Q, Schmidt S, Iwamoto Y, Sun Y, Cremer S, Hoyer FF, Iborra-Egea O, Muñoz-Guijosa C, Fei Ji F, Zhou B, Adams RH, Wythe JD, Hidalgo J, Watanabe H, Jung Y, van der Laan AM, Piek JJ, Kfoury Y, Désogère PA, Vinegoni C, Dutta P, Sadreyev RI, Caravan P, Bayes-Genis A, Libby P, Scadden DT, Lin CP Naxerova K, Swirski FK, Nahrendorf M Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease. Nat Cardiovasc Res. 2022;1:28–44 - PMID: 35747128 - PMCID: PMC9216333 - DOI: 10.1038/s44161-021-00002-8
McAlpine CS, Park J, Griciuc A, Kim E, Choi SH, Iwamoto Y, Kiss MG, Christie KA, Vinegoni C, Poller WC, Mindur JE, Chan CT, He S, Janssen H, Wong LP, Downey J, Singh S, Anzai A, Kahles F, Jorfi M, Feruglio PF, Sadreyev RI, Weissleder R, Kleinstiver BP, Nahrendorf M, Tanzi RE, Swirski FK Astrocytic interleukin-3 programs microglia and limits Alzheimer’s disease. Nature. 2021;595(7869):701-706 - PMID: 34262178 - PMCID: PMC8934148 - DOI: 10.1038/s41586-021-03734-6
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Other macro and mesoscopic imaging technique are pursued such as Born normalized Optical Projection Tomography with molecular contrast for ex-vivo small animal and whole organ imaging. This technique provides detailed tomographic reconstruction of morphology and molecular probes within tissue with resolution up to 5 microns in entire organs (e.g. heart, brain, kidney, etc) and can complement in-vivo imaging techniques such as FMT and MPM.
We are developing new applications for novel microscopy imaging systems and design and conduct cutting-edge experiments. In particular we are interested in providing new techniques for motion compensation for multiphoton microscopy in order to avoid artifacts due to respiratory and cardiac motion. Both hardware and software based approaches are conducted.
CSB has been named as one of the NCI Innovative Research in Cancer Nanotechnology (IRCNs). The IRCNs engage in directed, product-focused research that aims to translate cutting-edge science and technology into the next generation of diagnostic and therapeutic tools. These platforms serve as the core technologies for a wide array of specific applications that will ultimately benefit cancer patients.