Bouley, Richard, PhD

Assistant Professor

Phone: 617-724-9078
Email: Bouley.Richard@mgh.harvard.edu
Lab: Brown, Dennis

Publications

  • Cheung PW, Bouley R, Brown D Targeting the Trafficking of Kidney Water Channels for Therapeutic Benefit. Annu Rev Pharmacol Toxicol. 2020;60:175-194 - PMID: 31561739 - DOI: 10.1146/annurev-pharmtox-010919-023654

  • Nair AV, Yanhong W, Paunescu TG, Bouley R, Brown D Sex-dependent differences in water homeostasis in wild-type and V-ATPase B1-subunit deficient mice. PLoS ONE. 2019;14(8):e0219940 - PMID: 31386675 - PMCID: PMC6684071 - DOI: 10.1371/journal.pone.0219940

  • Arystarkhova E, Bouley R, Liu YB, Sweadner KJ Impaired AQP2 trafficking in Fxyd1 knockout mice: A role for FXYD1 in regulated vesicular transport. PLoS ONE. 2017;12(11):e0188006 - PMID: 29155857 - PMCID: PMC5695786 - DOI: 10.1371/journal.pone.0188006

  • He Q, Bouley R, Liu Z, Wein MN, Zhu Y, Spatz JM, Wang CY, Divieti Pajevic P, Plagge A, Babitt JL, Bastepe M Large G protein α-subunit XLαs limits clathrin-mediated endocytosis and regulates tissue iron levels in vivo. Proc Natl Acad Sci U S A. 2017;114(45):E9559-E9568 - PMID: 29078380 - PMCID: PMC5692581 - DOI: 10.1073/pnas.1712670114

  • Cheung PW, Ueberdiek L, Day J, Bouley R, Brown D Protein phosphatase 2C (PP2C) is responsible for VP-induced dephosphorylation of AQP2 serine 261. Am J Physiol Renal Physiol. 2017;:ajprenal.00004.2017 - PMID: 28381458 - PMCID: PMC5582913 - DOI: 10.1152/ajprenal.00004.2017

  • Cheung PW, Nomura N, Nair AV, Pathomthongtaweechai N, Ueberdiek L, Lu HA, Brown D, Bouley R EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol. 2016;27(10):3105-3116 - PMID: 27694161 - PMCID: PMC5042667 - DOI: 10.1681/ASN.2015080903

  • Chang SY, Lo CS, Zhao XP, Liao MC, Chenier I, Bouley R, Ingelfinger JR, Chan JS, Zhang SL Overexpression of angiotensinogen downregulates aquaporin 1 expression via modulation of Nrf2-HO-1 pathway in renal proximal tubular cells of transgenic mice. J Renin Angiotensin Aldosterone Syst. 2016;17(3):ePub - PMID: 27638854 - PMCID: PMC5843896 - DOI: 10.1177/1470320316668737

  • Cheung PW, Nomura N, Nair AV, Pathomthongtaweechai N, Ueberdiek L, Jenny Lu HA, Brown D, Bouley R EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol. 2016;:ePub - PMID: 26961350 - DOI: 10.1681/ASN.2015080903

  • Azroyan A, Cortez-Retamozo V, Bouley R, Liberman R, Ruan YC, Kiselev E, Jacobson KA, Pittet MJ, Brown D, Breton S Renal Intercalated Cells Sense and Mediate Inflammation via the P2Y14 Receptor. PLoS ONE. 2015;10(3):e0121419 - PMID: 25799465 - PMCID: PMC4370445 - DOI: 10.1371/journal.pone.0121419

  • Arystarkhova E, Ralph DL, Liu YB, Bouley R, McDonough AA, Sweadner KJ Paradoxical activation of the sodium chloride cotransporter (NCC) without hypertension in kidney deficient in a regulatory subunit of Na,K-ATPase, FXYD2. Physiol Rep. 2014;2(12):ePub - PMID: 25472608 - PMCID: PMC4332208 - DOI: 10.14814/phy2.12226

  • Lan YY, Londoño D, Bouley R, Rooney MS, Hacohen N Dnase2a Deficiency Uncovers Lysosomal Clearance of Damaged Nuclear DNA via Autophagy. Cell Rep. 2014;9(1):180-92 - PMID: 25284779 - PMCID: PMC4555847 - DOI: 10.1016/j.celrep.2014.08.074

  • Nomura N, Nunes P, Bouley R, Nair AV, Shaw S, Ueda E, Pathomthongtaweechai N, Lu HA, Brown D High throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concentration. Am J Physiol Cell Physiol. 2014;307(7):C597-605 - PMID: 24944200 - PMCID: PMC4187055 - DOI: 10.1152/ajpcell.00154.2014

  • Feinstein TN, Yui N, Webber MJ, Wehbi VL, Stevenson HP, King JD, Hallows KR, Brown D, Bouley R, Vilardaga JP Noncanonical control of vasopressin receptor type 2 signaling by retromer and arrestin. J Biol Chem. 2013;288(39):27849-60 - PMID: 23935101 - PMCID: PMC3784700 - DOI: 10.1074/jbc.M112.445098

  • Gurkar AU, Chu K, Raj L, Bouley R, Lee SH, Kim YB, Dunn SE, Mandinova A, Lee SW Identification of ROCK1 kinase as a critical regulator of Beclin1-mediated autophagy during metabolic stress. Nat Commun. 2013;4:2189 - PMID: 23877263 - PMCID: PMC3740589 - DOI: 10.1038/ncomms3189

  • Nunes P, Ernandez T, Roth I, Qiao X, Strebel D, Bouley R, Charollais A, Ramadori P, Foti M, Meda P, Féraille E, Brown D, Hasler U Hypertonic stress promotes autophagy and microtubule-dependent autophagosomal clusters. Autophagy. 2013;9(4):550-67 - PMID: 23380587 - PMCID: PMC3627670 - DOI: 10.4161/auto.23662

  • Yui N, Lu HJ, Bouley R, Brown D AQP2 is necessary for vasopressin- and forskolin-mediated filamentous actin depolymerization in renal epithelial cells. Biol Open. 2012;1(2):101-8 - PMID: 23213402 - PMCID: PMC3507199 - DOI: 10.1242/bio.2011042

  • Bouley R Corin: a key protein of an adaptive renal mechanism to respond to salt variation?. Kidney Int. 2012;82(1):7-8 - PMID: 22699378 - DOI: 10.1038/ki.2012.123

  • Brown D, Bouley R, Paunescu TG, Breton S, Lu HA New insights into the dynamic regulation of water and acid/base balance by renal epithelial cells. Am J Physiol Cell Physiol. 2012;302(10):C1421-33 - PMID: 22460710 - PMCID: PMC3362000 - DOI: 10.1152/ajpcell.00085.2012

  • Chen S, Webber MJ, Vilardaga JP, Khatri A, Brown D, Ausiello DA, Lin HY, Bouley R Visualizing Microtubule-Dependent Vasopressin Type 2 Receptor Trafficking Using a New High-Affinity Fluorescent Vasopressin Ligand. Endocrinology. 2011;152(10):3893-904 - PMID: 21828182 - PMCID: PMC3176653 - DOI: 10.1210/en.2011-1049

  • Li W, Zhang Y, Bouley R, Chen Y, Matsuzaki T, Nunes P, Hasler U, Brown D, Lu HA Simvastatin Enhances Aquaporin 2 Surface Expression and Urinary Concentration in Vasopressin Deficient Brattleboro Rats through Modulation of Rho GTPase. Am J Physiol Renal Physiol. 2011;301(2):F309-18 - PMID: 21511701 - PMCID: PMC3154588 - DOI: 10.1152/ajprenal.00001.2011

  • Xia Y, Babitt JL, Bouley R, Zhang Y, Silva ND, Chen S, Zhuang Z, Samad TA, Brenner GJ, Anderson JL, Hong CC, Schneyer AL, Brown D, Lin HY Dragon Enhances BMP Signaling and Increases Transepithelial Resistance in Kidney Epithelial Cells. J Am Soc Nephrol. 2010;21(4):666-77 - PMID: 20167703 - PMCID: PMC2844302 - DOI: 10.1681/ASN.2009050511

  • Bouley R, Palomino Z, Tang SS, Nunes P, Kobori H, Lu HA, Shum WW, Sabolic I, Brown D, Ingelfinger JR, Jung FF Angiotensin II and Hypertonicity Modulate Proximal Tubular Aquaporin 1 (AQP1) Expression. Am J Physiol Renal Physiol. 2009;297(6):F1575-86 - PMID: 19776169 - PMCID: PMC2801332 - DOI: 10.1152/ajprenal.90762.2008

  • Ferrandon S, Feinstein TN, Castro M, Wang B, Bouley R, Potts JT, Gardella TJ, Vilardaga JP Sustained cyclic AMP production by parathyroid hormone receptor endocytosis. Nat Chem Biol. 2009;5(10):734-42 - PMID: 19701185 - PMCID: PMC3032084 - DOI: 10.1038/nchembio.206

  • Van Hoek AN, Bouley R, Lu YX, Silberstein CM, Brown D, Wax MB, Patil RV Vasopressin-induced differential stimulation of AQP4 splice variants regulates the in-membrane assembly of orthogonal arrays. Am J Physiol Renal Physiol. 2009;296(6):F1396-404 - PMID: 19297454 - PMCID: PMC2692439 - DOI: 10.1152/ajprenal.00018.2009

  • Zalyapin EA, Bouley R, Hasler U, Vilardaga JP, Lin HY, Brown D, Ausiello DA Effects of the renal medullary pH and ionic environment on vasopressin binding and signaling. Kidney Int. 2008;74(12):1557-67 - PMID: 18813286 - PMCID: PMC3730289 - DOI: 10.1038/ki.2008.412

  • Nunes P, Hasler U, McKee M, Lu HA, Bouley R, Brown D A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2 (AQP2). Am J Physiol Cell Physiol. 2008;295(6):C1476-87 - PMID: 18799651 - PMCID: PMC2603565 - DOI: 10.1152/ajpcell.00344.2008

  • Brown D, Hasler U, Nunes P, Bouley R, Lu H Phosphorylation events and the modulation of aquaporin 2 cell surface expression. Curr Opin Nephrol Hypertens. 2008;17(5):491-8 - PMID: 18695390 - PMCID: PMC3774073 - DOI: 10.1097/MNH.0b013e3283094eb1

  • Hasler U, Nunes P, Bouley R, Lu HA, Matsuzaki T, Brown D Acute hypertonicity alters aquaporin-2 trafficking and induces a MAP kinase-dependent accumulation at the plasma membrane of renal epithelial cells. J Biol Chem. 2008;283(39):26643-61 - PMID: 18664568 - PMCID: PMC2546545 - DOI: 10.1074/jbc.M801071200

  • Bouley R, Hasler U, Lu HA, Nunes P, Brown D Bypassing vasopressin receptor signaling pathways in nephrogenic diabetes insipidus. Semin Nephrol. 2008;28:266-78 - PMID: 18519087 - PMCID: PMC2494582 - DOI: 10.1016/j.semnephrol.2008.03.010

  • Lu HA, Matsuzaki T, Bouley R, Hasler U, Qin Q, Brown D The phosphorylation state of serine 256 is dominant over that of serine 261 in the regulation of AQP2 trafficking in renal epithelial cells. Am J Physiol Renal Physiol. 2008;295(1):F290-4 - PMID: 18434387 - PMCID: PMC2494506 - DOI: 10.1152/ajprenal.00072.2008

  • Lu HA, Sun TX, Matsuzaki T, Yi XH, Eswara J, Bouley R, McKee M, Brown D Heat shock protein 70 interacts with aquaporin-2 (AQP2) and regulates its trafficking. J Biol Chem. 2007;282(39):28721-32 - PMID: 17636261 - DOI: 10.1074/jbc.M611101200

  • Yi X, Bouley R, Lin HY, Bechoua S, Sun TX, Del Re E, Shioda T, Raychowdhury MK, Lu HA, Abou-Samra AB, Brown D, Ausiello DA Alix (AIP1) is a vasopressin receptor (V2R)-interacting protein that increases lysosomal degradation of the V2R. Am J Physiol Renal Physiol. 2007;292(5):F1303-13 - PMID: 17287200 - DOI: 10.1152/ajprenal.00441.2005

  • Bouley R, Hawthorn G, Russo L, Lin H, Ausiello D, Brown D Aquaporin 2 (AQP2) and vasopressin type 2 receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in 'endocytosis-resistant' membrane domains after vasopressin treatment. Biol Cell. 2006;98(4):215-32 - PMID: 16563128 - DOI: 10.1042/BC20040054

  • Bouley R, Lin HY, Raychowdhury MK, Marshansky V, Brown D, Ausiello DA Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: involvement of a lysosomal degradation pathway. Am J Physiol Cell Physiol. 2005;288(6):C1390-401 - PMID: 15677378 - DOI: 10.1152/ajpcell.00353.2004

  • Bouley R, Hawthorn G, Russo L, Lin HY, Ausiello D, Brown D Aquaporin 2 (AQP2) and vasopressin receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in "endocytosis-resistant" membrane domains after vasopressin treatment. Abdom Imaging. 2005;:ePub - PMID: 16104839

  • Bouley R, Pastor-Soler N, Cohen O, McLaughlin M, Breton S, Brown D Stimulation of AQP2 membrane insertion in renal epithelial cells in vitro and in vivo by the cGMP phosphodiesterase inhibitor sildenafil citrate (Viagra). Am J Physiol Renal Physiol. 2005;288(6):F1103-12 - PMID: 15644488 - DOI: 10.1152/ajprenal.00337.2004

  • Stevens AL, Breton S, Gustafson CE, Bouley R, Nelson RD, Kohan DE, Brown D Aquaporin 2 is a vasopressin-independent, constitutive apical membrane protein in rat vas deferens. Am J Physiol Cell Physiol. 2000;278(4):C791-802 - PMID: 10751327 - DOI: 10.1152/ajpcell.2000.278.4.C791

  • Bouley R, Breton S, Sun T, McLaughlin M, Nsumu NN, Lin HY, Ausiello DA, Brown D Nitric oxide and atrial natriuretic factor stimulate cGMP-dependent membrane insertion of aquaporin 2 in renal epithelial cells. J Clin Invest. 2000;106(9):1115-26 - PMID: 11067864 - PMCID: PMC301414 - DOI: 10.1172/JCI9594