Brown, Dennis, PhD
Dennis Brown is a Ph. D. cell biologist who specializes in the use of state-of-the art fluorescence imaging and electron microscopy techniques to follow and dissect physiologically-relevant membrane protein trafficking events in epithelial and non-epithelial cells. He serves as the Associate Director of the CSB and the Director of the MGH Program in Membrane Biology (PMB) which uses use state of the art technologies in the pursuit of important biological questions at the system level. He became Professor of Medicine at Harvard in 2001 and has been at MGH since 1985.
Sensing, Signaling and Sorting Events in Kidney Epithelial Cell Physiology.
Segmental Expression of the Bradykinin Type 2 Receptor in Rat Efferent Ducts and Epididymis and Its Role in the Regulation of Aquaporin 9.
Angiotensin II and Hypertonicity Modulate Proximal Tubular Aquaporin 1 (AQP1) Expression.
Vasopressin-induced differential stimulation of AQP4 splice variants regulates the in-membrane assembly of orthogonal arrays.
Aquaporin 9 expression in the developing rat epididymis is modulated by steroid hormones.
Regulation of the V-ATPase in kidney epithelial cells: dual role in acid-base homeostasis and vesicle trafficking.
Regulation of luminal acidification in the male reproductive tract via cell-cell crosstalk.
Transepithelial Projections from Basal Cells are Luminal Sensors in Pseudostratified Epithelia
Impaired Tubular Uptake Explains Albuminuria in Early Diabetic Nephropathy.
The V-ATPase B1-subunit promoter drives expression of Cre recombinase in intercalated cells of the kidney.
Effects of the renal medullary pH and ionic environment on vasopressin binding and signaling.
Acute hypertonicity alters aquaporin-2 trafficking and induces a MAP kinase-dependent accumulation at the plasma membrane of renal epithelial cells.
The soluble transforming growth factor-beta receptor: Advantages and applications.
Phosphorylation events and the modulation of aquaporin 2 cell surface expression.
A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2 (AQP2).
V-ATPase expression in the mouse olfactory epithelium.
The phosphorylation state of serine 256 is dominant over that of serine 261 in the regulation of AQP2 trafficking in renal epithelial cells.
Aquaporins and vasopressin signaling in kidney health and disease: introduction.
Bypassing vasopressin receptor signaling pathways in nephrogenic diabetes insipidus.
Response to ''Albumin concentration in the Bowman's capsule: Multiphoton microscopy vs micropuncture technique''
Kidney Int. 2007;72:1411 - PMID: 18004315
Response to ''On the origin of albuminuria''
Kidney Int. 2007;72:1409-10 - PMID: 18004312
Controversies in nephrology: Response to ''renal albumin handling, facts, and artifacts''
The normal kidney filters nephrotic levels of albumin retrieved by proximal tubule cells: retrieval is disrupted in nephrotic states.
Evidence for a role of transforming growth factor (TGF)-beta1 in the induction of postglomerular albuminuria in diabetic nephropathy: amelioration by soluble TGF-beta type II receptor.
Role of NHERF1, CFTR and cAMP in the regulation of aquaporin 9
Heat shock protein 70 interacts with aquaporin-2 (AQP2) and regulates its trafficking.
Relocalization of the V-ATPase B2 subunit to the apical membrane of epididymal clear cells of mice deficient in the B1 subunit.
Compensatory membrane expression of the V-ATPase B2 subunit isoform in renal medullary intercalated cells of B1-deficient mice.
Association Of Soluble Adenylyl Cyclase (sAC) With The V-ATPase In Renal Epithelial Cells.
Alix (AIP1) is a vasopressin receptor (V2R)-interacting protein that increases lysosomal degradation of the V2R.
V-ATPase interacts with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway.
Segmental and cellular expression of aquaporins in the male excurrent duct.
New insights into the regulation of V-ATPase-dependent proton secretion.
Aquaporin 2 (AQP2) and vasopressin type 2 receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in 'endocytosis-resistant' membrane domains after vasopressin treatment.
TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function.
The B1-subunit of the H(+) ATPase is required for maximal urinary acidification.
Modulation of the actin cytoskeleton via gelsolin regulates vacuolar H+-ATPase recycling.
Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis.
Postnatal expression of aquaporins in epithelial cells of the rat epididymis.
Loss of basolateral invaginations in proximal tubules of cadmium-intoxicated rats is independent of microtubules and clathrin.
Defective thyroglobulin storage in LDL receptor-associated protein-deficient mice.
Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: involvement of a lysosomal degradation pathway.
V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice.
Stimulation of AQP2 membrane insertion in renal epithelial cells in vitro and in vivo by the cGMP phosphodiesterase inhibitor sildenafil citrate (Viagra).
Aquaporin 2 (AQP2) and vasopressin receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in "endocytosis-resistant" membrane domains after vasopressin treatment.
Abdom Imaging. 2005;:ePub - PMID: 16104839
Expression of the 56-kDa B2 subunit isoform of the vacuolar H(+)-ATPase in proton-secreting cells of the kidney and epididymis.
Renal vacuolar H+-ATPase.
A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse.
Bicarbonate-regulated adenylyl cyclase (sAC) is a sensor that regulates pH-dependent V-ATPase recycling.
Differential expression and targeting of endogenous Arf1 and Arf6 small GTPases in kidney epithelial cells in situ.
Detection of ClC-3 and ClC-5 in epididymal epithelium: immunofluorescence and RT-PCR after LCM.
Inhibition of endocytosis causes phosphorylation (S256)-independent plasma membrane accumulation of AQP2.
Hypertonicity is involved in redirecting the aquaporin-2 water channel into the basolateral, instead of the apical, plasma membrane of renal epithelial cells.
Expression of aquaporin 9 in the adult rat epididymal epithelium is modulated by androgens.
Physiological importance of endosomal acidification: potential role in proximal tubulopathies.
Curr Opin Nephrol Hypertens. 2002;11(5):527-37 - PMID: 12187318
Renal principal cell-specific expression of green fluorescent protein in transgenic mice.
Antigen retrieval reveals widespread basolateral expression of syntaxin 3 in renal epithelia.
Intra-endosomal pH-sensitive recruitment of the Arf-nucleotide exchange factor ARNO and Arf6 from cytoplasm to proximal tubule endosomes.
Aquaporin 9 expression along the male reproductive tract.
Distribution of the vacuolar H+ atpase along the rat and human male reproductive tract.
Remodeling the cellular profile of collecting ducts by chronic carbonic anhydrase inhibition.
Subcellular localization of presenilin 2 endoproteolytic C-terminal fragments.
Brain Res Mol Brain Res. 2001;96(1-2):14-20 - PMID: 11731004
Cytosolic phospholipase A(2) regulates golgi structure and modulates intracellular trafficking of membrane proteins.
Nitric oxide and atrial natriuretic factor stimulate cGMP-dependent membrane insertion of aquaporin 2 in renal epithelial cells.
Sorting proteins to their target membranes.
The B1 subunit of the H+ATPase is a PDZ domain-binding protein. Colocalization with NHE-RF in renal B-intercalated cells.
RGS4 and RGS2 bind coatomer and inhibit COPI association with Golgi membranes and intracellular transport.
Cadmium inhibits vacuolar H(+)ATPase-mediated acidification in the rat epididymis.
Aquaporin 2 is a vasopressin-independent, constitutive apical membrane protein in rat vas deferens.
Potassium depletion increases proton pump (H(+)-ATPase) activity in intercalated cells of cortical collecting duct.
Tetanus toxin-mediated cleavage of cellubrevin inhibits proton secretion in the male reproductive tract.
H(+)V-ATPase-dependent luminal acidification in the kidney collecting duct and the epididymis/vas deferens: vesicle recycling and transcytotic pathways.
J Exp Biol. 2000;203(Pt 1):137-45 - PMID: 10600682
Na/K-ATPase in intercalated cells along the rat nephron revealed by antigen retrieval.
J Am Soc Nephrol. 1999;10(5):913-22 - PMID: 10232676
Gene therapy of inherited renal tubular disease.
Localization of sodium bicarbonate cotransporter (NBC) protein and messenger ribonucleic acid in rat epididymis.
Postnatal development of H+ ATPase (proton-pump)-rich cells in rat epididymis.
Histochem Cell Biol. 1999;111(2):97-105 - PMID: 10090570
Cold-induced microtubule disruption and relocalization of membrane proteins in kidney epithelial cells.
J Am Soc Nephrol. 1998;9(2):155-66 - PMID: 9527391
Polarity, integrin, and extracellular matrix dynamics in the postischemic rat kidney.
Proton secretion in the male reproductive tract: involvement of Cl--independent HCO 3 - transport.
Proton secretion in the male reproductive tract: involvement of Cl--independent HCO-3 transport.
Basolateral distribution of caveolin-1 in the kidney. Absence from H+-atpase-coated endocytic vesicles in intercalated cells.
Polarized expression of membrane proteins in renal epithelial cells: involvement of specialized transport vesicles and intracellular pathways.
Adv Nephrol Necker Hosp. 1998;27:297-315 - PMID: 9408453
Immunolocalization of AE2 anion exchanger in rat kidney.
Localization of the high-affinity glutamate transporter EAAC1 in rat kidney.
Slit diaphragm-reactive nephritogenic MAb 5-1-6 alters expression of ZO-1 in rat podocytes.
Redistribution of villin to proximal tubule basolateral membranes after ischemia and reperfusion.
Role of V-ATPase-rich cells in acidification of the male reproductive tract.
J Exp Biol. 1997;200(Pt 2):257-62 - PMID: 9050233
Presence de cellules acidifiantes dans l'epididyme et le canal deferent: implication de la pompe a protons
Acidification of the male reproductive tract by a proton pumping (H+)-ATPase.
Nature Med. 1996;2(4):470-2 - PMID: 8597961
Mitochondria-rich, proton-secreting epithelial cells.
J Exp Biol. 1996;199(Pt 11):2345-58 - PMID: 9114500
Depletion of intercalated cells from collecting ducts of carbonic anhydrase II-deficient (CAR2 null) mice.