Pittet

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Pittet, Mikael, PhD

Mikael_pittet
Phone: 617-643-6285
Email: mpittet@mgh.harvard.edu

Research

Samana Cay MGH Research Scholar

For the past >20 years I have performed clinical and/or experimental investigations of immune responses in several inflammatory disorders including cancer, atherosclerosis, myocardial infarction and asthma. In my PhD work at the Ludwig Institute for Cancer Research, Switzerland (with Drs Jean-Charles Cerottini and Pedro Romero) I participated in the identification ex vivo of anti-tumor T cells in human patients with cancer. These studies have shown that tumor-specific T cells frequently accumulate at tumor sites but on account of their anergic state fail to control tumor growth. I then joined the Center for Molecular Imaging Research at MGH as a postdoctoral fellow (directed by Dr Ralph Weissleder), established ties with immunology laboratories at Harvard Medical School (Drs. Harald von Boehmer, Khashayarsha Khazaie and Ulrich von Andrian) and used intravital imaging modalities to assess tumor-specific T cell immune responses directly in vivo. The studies identified key roles for TGF-beta signaling and for T regulatory cells in the inhibition of cytotoxic T cell responses.

My laboratory, established in 2007, performs a research program on the host immune response in vivo. We aim to understand better where, when and how immune cells are produced, traffic, and mediate regulatory or effector functions. For example, our recent work has identified new information on the origins, mobilization and amplification of innate immune cells known as monocytes. We have also started to define some aspects of the functional relevance of these cells in enhancing or suppressing inflammation. Our next goals consist to dissect more intimately the responses mediated by monocytes and other host cells in response to various forms of cancers or other inflammatory diseases. Experimental and clinical evidence indicates that diverse populations of immune cells infiltrate the tumor stroma and enhance cancer progression. We aim not only to understand how tumors are able to co-opt tumor-promoting immune cells––both locally and remotely––but also whether new cancer molecular and cellular targets may prove valuable in the diagnosis and treatment of the disease.

To contribute a more dynamic understanding of immune reactions in the body, we examine the behavior and functions of cells by combining several experimental tools. These include classic molecular and cellular techniques, microsurgical procedures and in vivo imaging modalities. In vitro readouts do not always recapitulate the complexity of in vivo environments, thus we use real-time imaging technologies, which permit to assess the immune system quantitatively and in living subjects. We collaborate with various CSB investigators and with other laboratories in Boston, the U.S., and abroad.

Selected Publications (from total of 116)

Arlauckas SP*, Garris CS*, Kohler RH, Kitaoka M, Cuccarese MF, Yang KS, Miller MA, Carlson JC, Freeman GJ, Anthony RM, Weissleder R, Pittet MJ
In vivo imaging reveals a tumor-associated macrophage–mediated resistance pathway in anti–PD-1 therapy
Sci Transl Med. 2017;9(389):eaal3604 - PMID: 28490665 - Cover
Pucci F, Rickelt S, Newton AP, Garris C, Nunes E, Evavold C, Pfirschke C, Engblom C, Mino-Kenudson M, Hynes RO, Weissleder R, Pittet MJ
PF4 Promotes Platelet Production and Lung Cancer Growth.
Cell Rep. 2016;17(7):1764-1772 - PMID: 27829148 - PMCID: PMC5108525
Engblom C, Pfirschke C, Pittet MJ
The role of myeloid cells in cancer therapies.
Nat Rev Cancer. 2016;16(7):447-62 - PMID: 27339708
Pucci F, Garris C, Lai CP, Newton A, Pfirschke C, Engblom C, Alvarez D, Sprachman M, Evavold C, Magnuson A, von Andrian UH, Glatz K, Breakefield XO, Mempel TR, Weissleder R, Pittet MJ
SCS macrophages suppress melanoma by restricting tumor-derived vesicle–B cell interactions
Science. 2016;352(6282):242-246 - PMID: 26989197 - PMCID: PMC4960636
Pfirschke C, Engblom C, Rickelt S, Cortez-Retamozo V, Garris C, Pucci F, Yamasaki T, Poirier-Colame V, Newton A, Redouane Y, Lin YJ, Wojtkiewicz G, Iwamoto Y, Mino-Kenudson M, Huynh TG, Hynes RO, Freeman GJ Kroemer G, Zitvogel L, Weissleder R, Pittet MJ
Immunogenic Chemotherapy Sensitizes Tumors to Checkpoint Blockade Therapy
Immunity. 2016;44:1-12 - PMID: 26872698 - PMCID: PMC4758865
Weissleder R, Nahrendorf M, Pittet MJ
Imaging macrophages with nanoparticles.
Nat Mater. 2014;13(2):125-38 - PMID: 24452356 - Cover
Bronte V, Pittet MJ
The spleen in local and systemic regulation of immunity.
Immunity. 2013;39(5):806-18 - PMID: 24238338 - PMCID: PMC3912742
Cortez-Retamozo V, Etzrodt M, Newton A, Ryan R, Pucci F, Sio SW, Kuswanto W, Rauch PJ, Chudnovskiy A, Iwamoto Y, Kohler R, Marinelli B, Gorbatov R, Wojtkiewicz G, Panizzi P, Mino-Kenudson M, Forghani R, Figueiredo JL, Chen JW, Xavier R, Swirski FK, Nahrendorf M, Weissleder R, Pittet MJ
Angiotensin II Drives the Production of Tumor-Promoting Macrophages
Immunity. 2013;38(2):296-308 - PMID: 23333075 - PMCID: PMC3582771
Cortez-Retamozo V, Etzrodt M, Newton A, Rauch PJ, Chudnovskiy A, Berger C, Ryan RJ, Iwamoto Y, Marinelli B, Gorbatov R, Forghani R, Novobrantseva TI, Koteliansky V, Figueiredo JL, Chen JW, Anderson DG, Nahrendorf M, Swirski FK, Weissleder R, Pittet MJ
Origins of tumor-associated macrophages and neutrophils.
Proc Natl Acad Sci U S A. 2012;109(7):2491-6 - PMID: 22308361 - PMCID: PMC3289379
Pittet MJ, Weissleder R
Intravital imaging.
Cell. 2011;147(5):983-91 - PMID: 22118457 - PMCID: PMC3824153
Swirski* FK, Nahrendorf* M, Etzrodt M, Wildgruber M, Cortez-Retamozo V, Panizzi P, Figueiredo JL, Kohler RH, Chudnovskiy A, Waterman P, Aikawa E, Mempel TR, Libby P, Weissleder R, Pittet MJ
Identification of Splenic Reservoir Monocytes and Their Deployment to Inflammatory Sites
Science. 2009;325 (5940):612-6 - PMID: 19644120 - PMCID: PMC2803111
Weissleder R, Pittet MJ
Imaging in the era of molecular oncology
Nature. 2008;452:580-589 - PMID: 18385732 - PMCID: PMC2708079
Nahrendorf M, Swirski FK, Aikawa E, Stangenberg L, Wurdinger T, Figueiredo JL, Libby P, Weissleder R, Pittet MJ
The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.
J Exp Med. 2007;204(12):3037-47 - PMID: 18025128 - PMCID: PMC2118517
Swirski FK, Libby P, Aikawa E, Alcaide P, Luscinskas FW, Weissleder R, Pittet MJ
Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromata
J Clin Invest. 2007;117(1):195-205 - PMID: 17200719 - PMCID: PMC1716211
Zippelius A, Batard P, Rubio-Godoy V, Bioley G, Lienard D, Lejeune F, Rimoldi D, Guillaume P, Meidenbauer N, Mackensen A, Rufer N, Lubenow N, Speiser D, Cerottini J, Romero P, Pittet M
Effector function of human tumor-specific CD8 T cells in melanoma lesions: a state of local functional tolerance.
Cancer Res. 2004;64:2865-73 - PMID: 15087405
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