Miller, Miles , PhD

Assistant Professor

Lab: Miller, Miles

Miles Miller received his A.B. in Chemistry from Princeton University, and his Ph.D. in Biological Engineering from the Massachusetts Institute of Technology (Advisors: Douglas Lauffenburger and Linda Griffith). He trained as a NIH postdoctoral fellow under Dr. Ralph Weissleder at MGH. He specializes in parsing mechanisms of cell signaling and drug action from a quantitative network-level perspective, with training in computational modeling, imaging, nanotechnology, cell signaling biology, and drug delivery.


  • Baskaran JP, Weldy A, Guarin J, Munoz G, Shpilker PH, Kotlik M, Subbiah N, Wishart A, Peng Y, Miller MA, Cowen L, Oudin MJ Cell shape, and not 2D migration, predicts extracellular matrix-driven 3D cell invasion in breast cancer. APL Bioeng. 2020;4(2):026105 - PMID: 32455252 - PMCID: PMC7202897 - DOI: 10.1063/1.5143779

  • Wang SJ, Ran Li R, Ng TSC, Luthria G, Oudin MJ, Prytyskach M, Kohler RH, Weissleder R, Lauffenburger DA, Miller MA Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy. Sci Adv. 2020;6(21):eaaz8521 - DOI: 10.1126/sciadv.aaz8521

  • Li R, Ng TSC, Garlin MA, Weissleder R*, Miller MA* Understanding the In Vivo Fate of Advanced Materials by Imaging. Adv Funct Mater. 2020;:1910369 - DOI: 10.1002/adfm.201910369

  • Li R, Attari A, Prytyskach M, Garlin MA, Weissleder R, Miller MA Single‐Cell Intravital Microscopy of Trastuzumab Quantifies Heterogeneous in vivo Kinetics. Cytometry A. 2020;97(5):528-539 - PMID: 31423731 - PMCID: PMC7028488 - DOI: 10.1002/cyto.a.23872

  • Ng TSC, Garlin MA, Weissleder R, Miller MA Understanding the EPR effect through image-guided systems pharmacology. Theranostics. 2019;:ePub

  • Ahrens CC, Chiswick EL, Ravindra KC, Miller MA, Ramseier J, Isaacson KB, Lauffenburger DA, Griffith LG Development and Application of the Metalloprotease Activity Multi-plexed Bead-based Immunoassay (MAMBI). Biochemistry. 2019;58(38):3938-3942 - PMID: 31474112 - DOI: 10.1021/acs.biochem.9b00584

  • Ng EX, Sun G, Wei SC, Miller MA, Dasgupta R, Lam PYP, Chen CH Ultrafast Single-Cell Level Enzymatic Tumor Profiling. Anal Chem. 2019;91(2):1277-1285 - PMID: 30362713 - DOI: 10.1021/acs.analchem.8b02576

  • Miller MA, Mikula H, Luthria G, Li R, Kronister S, Prytyskach M, Kohler RH, Mitchison T, Weissleder R Modular Nanoparticulate Prodrug Design Enables Efficient Treatment of Solid Tumors Using Bioorthogonal Activation. ACS Nano. 2018;12(12):12814-12826 - PMID: 30550257 - PMCID: PMC6307086 - DOI: 10.1021/acsnano.8b07954

  • Kim HY, Li R, Ng TSC, Courties G, Rodell CB, Prytyskach M, Kohler RH, Pittet M, Nahrendorf M, Weissleder R, Miller MA Quantitative Imaging of Tumor Associated Macrophages and Their Response to Therapy Using 64Cu-Labeled Macrin. ACS Nano. 2018;12(12):12015-12029 - PMID: 30508377 - PMCID: PMC6482841 - DOI: 10.1021/acsnano.8b04338

  • Pineda JJ, Miller MA, Song Y, Kuhn H, Mikula H, Tallapragada N, Weissleder R, Mitchison TJ Site occupancy calibration of taxane pharmacology in live cells and tissues. Proc Natl Acad Sci U S A. 2018;115(48):E11406-E11414 - PMID: 30429313 - PMCID: PMC6275538 - DOI: 10.1073/pnas.1800047115

  • Yoneyama T, Gorry M, Sobo-Vujanovic A, Lin Y, Vujanovic L, Gaither-Davis A, Moss ML, Miller MA, Griffith LG, Lauffenburger DA, Stabile LP, Herman J, Vujanovic NL ADAM10 Sheddase Activity is a Potential Lung-Cancer Biomarker. J Cancer. 2018;9(14):2559-2570 - PMID: 30026855 - PMCID: PMC6036891 - DOI: 10.7150/jca.24601

  • Miller MA Gold photothermal therapy: A positive for negative margins. Sci Transl Med. 2018;10(423):eaar7512 - DOI: 10.1126/scitranslmed.aar7512

  • Yoneyama T, Gorry M, Miller MA, Gaither-Davis A, Lin Y, Moss ML, Griffith LG, Lauffenburger DA, Stabile LP, Herman JG, Vujanovic NL Modification of proteolytic activity matrix analysis (PrAMA) to measure ADAM10 and ADAM17 sheddase activities in cell and tissue lysates. J Cancer. 2017;8(19):3916-3932 - PMID: 29187866 - PMCID: PMC5705993 - DOI: 10.7150/jca.20779

  • Miller MA, Kim E, Cuccarese MF, Plotkin AL, Prytyskach M, Kohler RH, Pittet MJ, Weissleder R Near infrared imaging of Mer tyrosine kinase (MERTK) using MERi-SiR reveals tumor associated macrophage uptake in metastatic disease. Chem Commun. 2017;54(1):42-45 - PMID: 29185561 - PMCID: PMC5736449 - DOI: 10.1039/c7cc07581b

  • Miller MA Less is more for anticancer therapy combinations. Sci Transl Med. 2017;9 (411):aap8166 - DOI: 10.1126/scitranslmed.aap8166

  • Miller MA It’s what’s inside that counts for nanoparticle vaccines. Sci Transl Med. 2017;9(417):eaar2438 - DOI: 10.1126/scitranslmed.aar2438

  • Miller MA Counting on carbon quantum dots to clear infection. Sci Transl Med. 2017;9(399):eaao0969

  • Miller MA Nanotransfection brings progress that’s more than skin-deep. Sci Transl Med. 2017;9(405):eaao4216

  • Miller MA, Weissleder R Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior. Adv Drug Deliv Rev. 2017;113:61-86 - PMID: 27266447 - PMCID: PMC5136524 - DOI: 10.1016/j.addr.2016.05.023

  • Conrad C, Dorzweiler K, Miller MA, Lauffenburger DA, Strik H, Bartsch JW Profiling of metalloprotease activities in cerebrospinal fluids of patients with neoplastic meningitis. Fluids Barriers CNS. 2017;14(1):22 - PMID: 28806983 - PMCID: PMC5556623 - DOI: 10.1186/s12987-017-0070-5

  • Miller MA, Askevold B, Mikula H, Kohler RH, Pirovich D, Weissleder R Nano-palladium is a cellular catalyst for in vivo chemistry. Nat Commun. 2017;8:15906 - PMID: 28699627 - PMCID: PMC5510178 - DOI: 10.1038/ncomms15906

  • Miller MA, Arlauckas S, Weissleder R Prediction of Anti-cancer Nanotherapy Efficacy by Imaging. Nanotheranostics. 2017;1(3):296-312 - PMID: 29071194 - PMCID: PMC5646731 - DOI: 10.7150/ntno.20564

  • Miller MA, Weissleder R Imaging of anticancer drug action in single cells. Nat Rev Cancer. 2017;17(7):399–414 - PMID: 28642603 - DOI: 10.1038/nrc.2017.41

  • Miller MA Driver mutations take the wheel in invasive yet nonmalignant disease. Sci Transl Med. 2017;9(393):ePub - PMID: 28592567 - DOI: 10.1126/scitranslmed.aan8194

  • Miller MA, Chandra R, Cuccarese MF, Pfirschke C, Engblom C, Stapleton S, Adhikary U, Kohler RH, Mohan JF, Pittet MJ, Weissleder R Radiation therapy primes tumors for nanotherapeutic delivery via macrophage-mediated vascular bursts. Sci Transl Med. 2017;9(392):eaal0225 - PMID: 28566423 - PMCID: PMC6681815 - DOI: 10.1126/scitranslmed.aal0225

  • Arlauckas SP*, Garris CS*, Kohler RH, Kitaoka M, Cuccarese MF, Yang KS, Miller MA, Carlson JC, Freeman GJ, Anthony RM, Weissleder R, Pittet MJ In vivo imaging reveals a tumor-associated macrophage–mediated resistance pathway in anti–PD-1 therapy. Sci Transl Med. 2017;9(389):eaal3604 - PMID: 28490665 - PMCID: PMC5734617 - DOI: 10.1126/scitranslmed.aal3604

  • Oudin MJ, Barbier L, Schäfer C, Kosciuk T, Miller MA, Han S, Jonas O, Lauffenburger DA, Gertler FB MENA Confers Resistance to Paclitaxel in Triple-Negative Breast Cancer. Mol Cancer Ther. 2017;16(1):143-155 - PMID: 27811011 - PMCID: PMC5359014 - DOI: 10.1158/1535-7163.MCT-16-0413

  • Miller MA Nanoparticles improve economic mileage for CARs. Sci Transl Med. 2017;9(387):ePub - PMID: 28446684 - DOI: 10.1126/scitranslmed.aan2784

  • Conrad C, Miller MA, Bartsch JW, Schlomann U, Lauffenburger DA Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method. Methods Mol Biol. 2017;1574:243-253 - PMID: 28315256 - DOI: 10.1007/978-1-4939-6850-3_18

  • Miller MA Richer data with personalized GEMs. Sci Transl Med. 2017;9(381):ePub - PMID: 28298424 - DOI: 10.1126/scitranslmed.aam9865

  • Cuccarese MF, Dubach JM, Pfirschke C, Engblom C, Garris C, Miller MA, Pittet MJ, Weissleder R Heterogeneity of macrophage infiltration and therapeutic response in lung carcinoma revealed by 3D organ imaging. Nat Commun. 2017;8:14293 - PMID: 28176769 - PMCID: PMC5309815 - DOI: 10.1038/ncomms14293

  • Miller MA, Sullivan RJ, Lauffenburger DA Molecular Pathways: Receptor Ectodomain Shedding in Treatment, Resistance, and Monitoring of Cancer. Clin Cancer Res. 2017;23(3):623-629 - PMID: 27895032 - PMCID: PMC5290119 - DOI: 10.1158/1078-0432.CCR-16-0869

  • Miller MA, Weissleder R Imaging the pharmacology of nanomaterials by intravital microscopy: Towards understanding their biological behavior. Adv Drug Deliv Rev. 2017;113:61-86 - PMID: 27266447 - PMCID: PMC5136524 - DOI: 10.1016/j.addr.2016.05.023

  • Ng EX, Miller MA, Jing T, Chen CH Single cell multiplexed assay for proteolytic activity using droplet microfluidics. Biosens Bioelectron. 2016;81:408-14 - PMID: 26995287 - DOI: 10.1016/j.bios.2016.03.002

  • Moss ML, Miller MA, Vujanovic N, Yoneyama T, Rasmussen FH Fluorescent substrates for ADAM15 useful for assaying and high throughput screening. Anal Biochem. 2016;514:42-47 - PMID: 27637923 - DOI: 10.1016/j.ab.2016.09.010

  • Oudin MJ, Miller MA, Klazen JA, Kosciuk T, Lussiez A, Hughes SK, Tadros J, Bear JE, Lauffenburger DA, Gertler FB MenaINV mediates synergistic crosstalk between signaling pathways driving chemotaxis and haptotaxis. Mol Biol Cell. 2016;27(20):3085-3094 - PMID: 27559126 - PMCID: PMC5063616 - DOI: 10.1091/mbc.E16-04-0212

  • Miller MA, Oudin MJ, Sullivan RJ, Wang SJ, Meyer AS, Im H, Frederick DT, Tadros J, Griffith LG, Lee H, Weissleder R, Flaherty KT, Gertler FB, Lauffenburger DA Reduced Proteolytic Shedding of Receptor Tyrosine Kinases Is a Post-Translational Mechanism of Kinase Inhibitor Resistance. Cancer Discov. 2016;6(4):382-99 - PMID: 26984351 - PMCID: PMC5087317 - DOI: 10.1158/2159-8290.CD-15-0933

  • Miller MA, Moss ML, Powell G, Petrovich R, Edwards L, Meyer AS, Griffith LG, Lauffenburger DA Targeting autocrine HB-EGF signaling with specific ADAM12 inhibition using recombinant ADAM12 prodomain. Sci Rep. 2015;5:15150 - PMID: 26477568 - PMCID: PMC4609913 - DOI: 10.1038/srep15150

  • Schlomann U, Koller G, Conrad C, Ferdous T, Golfi P, Garcia AM, Höfling S, Parsons M, Costa P, Soper R, Bossard M, Hagemann T, Roshani R, Sewald N, Ketchem RR, Moss ML, Rasmussen FH, Miller MA, Lauffenburger DA, Tuveson DA, Nimsky C, Bartsch JW ADAM8 as a drug target in pancreatic cancer. Nat Commun. 2015;6:6175 - PMID: 25629724 - PMCID: PMC5014123 - DOI: 10.1038/ncomms7175

  • Ng EX, Miller MA, Jing T, Lauffenburger DA, Chen CH Low-volume multiplexed proteolytic activity assay and inhibitor analysis through a pico-injector array. Lab Chip. 2015;15(4):1153-9 - PMID: 25553996 - DOI: 10.1039/c4lc01162g

  • Miller MA, Gadde S, Pfirschke C, Engblom C, Sprachman MM, Kohler RH, Yang KS, Laughney AM, Wojtkiewicz G, Kamal N, Bhonagiri S, Pittet MJ, Farokhzad OC, Weissleder R Predicting therapeutic nano-medicine efficacy using a companion MR imaging nanoparticle. Sci Transl Med. 2015;7:314 - PMID: 26582898 - PMCID: PMC5462466 - DOI: 10.1126/scitranslmed.aac6522

  • Miller MA, Zheng YR, Gadde S, Pfirschke C, Zope H, Engblom C, Kohler RH, Iwamoto Y, Yang KS, Askevold B, Kolishetti N, Pittet M, Lippard SJ, Farokhzad OC, Weissleder R Tumour-associated macrophages act as a slow-release reservoir of nano-therapeutic Pt(IV) pro-drug. Nat Commun. 2015;6:8692 - PMID: 26503691 - PMCID: PMC4711745 - DOI: 10.1038/ncomms9692

  • Vinegoni C, Dubach JM, Thurber GM, Miller MA, Mazitschek R, Weissleder R Advances in measuring single-cell pharmacokinetics and pharmacology in vivo. Drug Discov Today. 2015;20(9):1087-92 - PMID: 26024776 - PMCID: PMC4567932 - DOI: 10.1016/j.drudis.2015.05.011

  • Laughney AM, Kim E, Sprachman MM, Miller MA, Kohler RH, Yang KS, Orth JD, Mitchison TJ, Weissleder R Single-cell pharmacokinetic imaging reveals a therapeutic strategy to overcome drug resistance to the microtubule inhibitor eribulin. Sci Transl Med. 2014;6 (261):261ra152 - PMID: 25378644 - PMCID: PMC4330962 - DOI: 10.1126/scitranslmed.3009318

  • Turetsky A, Kim E, Kohler RH, Miller MA, Weissleder R Single cell imaging of Bruton's tyrosine kinase using an irreversible inhibitor. Sci Rep. 2014;4:4782 - PMID: 24759210 - PMCID: PMC3998017 - DOI: 10.1038/srep04782

  • Miller MA, Askevold B, Yang KS, Kohler RH, Weissleder R Platinum Compounds for High-Resolution In Vivo Cancer Imaging. ChemMedChem. 2014;9(6):1131-5 - PMID: 24504646 - PMCID: PMC4037345 - DOI: 10.1002/cmdc.201300502

  • Meyer AS, Miller MA, Gertler FB, Lauffenburger DA The receptor AXL diversifies EGFR signaling and limits the response to EGFR-targeted inhibitors in triple-negative breast cancer cells. Sci Signal. 2013;6(287):ra66 - PMID: 23921085 - PMCID: PMC3947921 - DOI: 10.1126/scisignal.2004155

  • Dang M, Armbruster N, Miller MA, Cermeno E, Hartmann M, Bell GW, Root DE, Lauffenburger DA, Lodish HF, Herrlich A Regulated ADAM17-dependent EGF family ligand release by substrate-selecting signaling pathways. Proc Natl Acad Sci U S A. 2013;110(24):9776-81 - PMID: 23720309 - PMCID: PMC3683718 - DOI: 10.1073/pnas.1307478110

  • Miller MA, Meyer AS, Beste MT, Lasisi Z, Reddy S, Jeng KW, Chen CH, Han J, Isaacson K, Griffith LG, Lauffenburger DA ADAM-10 and -17 regulate endometriotic cell migration via concerted ligand and receptor shedding feedback on kinase signaling. Proc Natl Acad Sci U S A. 2013;110(22):E2074-83 - PMID: 23674691 - PMCID: PMC3670354 - DOI: 10.1073/pnas.1222387110

  • Chen CH, Miller MA, Sarkar A, Beste MT, Isaacson KB, Lauffenburger DA, Griffith LG, Han J Multiplexed protease activity assay for low-volume clinical samples using droplet-based microfluidics and its application to endometriosis. J Am Chem Soc. 2013;135(5):1645-8 - PMID: 23157326 - PMCID: PMC3566300 - DOI: 10.1021/ja307866z

  • Miller MA, Feng XJ, Li G, Rabitz HA Identifying biological network structure, predicting network behavior, and classifying network state with High Dimensional Model Representation (HDMR). PLoS ONE. 2012;7(6):e37664 - PMID: 22723838 - PMCID: PMC3377689 - DOI: 10.1371/journal.pone.0037664

  • Miller M, Hafner M, Sontag E, Davidsohn N, Subramanian S, Purnick PE, Lauffenburger D, Weiss R Modular design of artificial tissue homeostasis: robust control through synthetic cellular heterogeneity. PLoS Comput Biol. 2012;8(7):e1002579 - PMID: 22829755 - PMCID: PMC3400602 - DOI: 10.1371/journal.pcbi.1002579

  • Moss ML, Powell G, Miller MA, Edwards L, Qi B, Sang QX, De Strooper B, Tesseur I, Lichtenthaler SF, Taverna M, Zhong JL, Dingwall C, Ferdous T, Schlomann U, Zhou P, Griffith LG, Lauffenburger DA, Petrovich R, Bartsch JW ADAM9 inhibition increases membrane activity of ADAM10 and controls α-secretase processing of amyloid precursor protein. J Biol Chem. 2011;286(47):40443-51 - PMID: 21956108 - PMCID: PMC3220463 - DOI: 10.1074/jbc.M111.280495

  • Chen CH, Sarkar A, Song YA, Miller MA, Kim SJ, Griffith LG, Lauffenburger DA, Han J Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator. J Am Chem Soc. 2011;133(27):10368-71 - PMID: 21671557 - PMCID: PMC3165005 - DOI: 10.1021/ja2036628

  • Miller MA, Barkal L, Jeng K, Herrlich A, Moss M, Griffith LG, Lauffenburger DA Proteolytic Activity Matrix Analysis (PrAMA) for simultaneous determination of multiple protease activities. Integr Biol (Camb). 2011;3(4):422-38 - PMID: 21180771 - PMCID: PMC3173501 - DOI: 10.1039/c0ib00083c

  • Miller M, Feng X, Li G, Rabitz H Nonlinear bionetwork structure inference using the random sampling-high dimensional model representation (RS-HDMR) algorithm. Conf Proc IEEE Eng Med Biol Soc. 2009;2009:6412-5 - PMID: 19964421 - DOI: 10.1109/IEMBS.2009.5333798