2010-02-01 We are looking to recruit a highly motivated postdoctoral fellow to join a very dynamic research effort focussing on basic cardiovascular biology. The laboratory is part of the Center for Systems Biology, one of 5 thematic centers at the MGH and of the Center for Molecular Imaging Research, both headed by Dr. Ralph Weissleder. Research interests of our group focus on 1) in vivo siRNA delivery to modulate innate immune responses, 2) imaging of molecular processes during the healing phase after myocardial infarction, 3) imaging of heart transplant rejection, 4) imaging of inflammation in atherosclerotic plaques. Imaging targets are enzymes, innate immune cells and molecular players with a central role in cardiovascular disease. We use the entire spectrum of modalities, including MRI, nuclear and optical imaging techniques. These technologies are embedded in a biologically driven research program that aims at systematic understanding of inflammation and repair at a basic level while keeping a rigorous translational perspective.</p> <p>Recent publications: Swirski FK*, Nahrendorf M* et al. Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science 2009, July 31.</p> <p>Nahrendorf M*, Swirski FK* et al. The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions. J Exp Med 2007;204:3037-47.</p> <p>Nahrendorf M et al. Nanoparticle PET-CT imaging of macrophages in inflammatory atherosclerosis. Circulation 2008;117:379-87.</p> 24 44 Postdoctoral Research Fellow Requirements: Experience in flow cytometry, cell culture and molecular biology techniques as well as an interest in innate immunity, imaging and cardiovascular pathophysiology. Successful applicants have a strong publication record, creative thinking and good team spirit. Due to funding restrictions, only US citizens, green card or VISA holder will be considered. Cardiovascular Molecular Imaging After a training period, the successful applicant will self-reliantly plan and conduct experiments and will be involved in writing scientific manuscripts. Full Time 2010-02-01 Several post-doctoral position are available in the laboratory of Ralph Weissleder, MD, PhD, Director of the MGH Center for Systems Biology. The laboratory’s overall goal is to understand how human biology and disease unfolds in vivo. We utilize unbiased approaches such as high-throughput chemical screens and profiling, combined with advanced imaging capabilities and traditional molecular and cell biology techniques. Available positions are: chip based detection cancer cells (Nat Med. 2008;14(8):869-74) nanomaterials and bioconjugation (Angew Chem Int Edit. 2009;48:5657-60) chemical biology to develop imaging agents (Nature 2008;452:580-589) mathematical modeling of cell trafficking (Science. 2009;325:612-6) intravital imaging of mitosis and apoptosis in cancer (Nature 2008;452:580-589) The Center for Systems Biology is located within the new Simches building on the main MGH campus, and features a dynamic, multi-disciplinary environment with close interactions with MGH clinician-researchers and investigators across Harvard and MIT. The Fellow will have simultaneous academic appointments at the Massachusetts General Hospital and Harvard Medical School. 28 1 Postdoctoral Research Fellow M.D. or Ph.D. degree, or students in their final year of their Ph.D. studies. Experience in any of the following disciplines is strongly preferred: cancer biology, cell biology, microscopy, immunology bioinformatics, mathematical modeling. U.S. citizen or permanent resident (green card) and outstanding publication record required. Candidates should include an updated CV, complete contact information for three references, and a cover letter describing their past research experience, career goals and potential future research interests. Systems Biology, Nanotechnology Full Time 2010-02-01 A Technologist position in the field of automated microscopy is available in the MGH Center for Systems Biology (CSB). CSB is located within the new Simches building on the main MGH campus, and features a dynamic, multi-disciplinary environment with close interactions with collaborators throughout the MGH and Broad Institute communities. 26 471 Research Technologist A master's degree in cell biology, molecular biology, chemistry, or related field, OR at least 2 years of full-time postgraduate experience in a research laboratory. Must have prior working experience with fluorescent microscopy, Cellular and Molecular Biology, mammalian tissue culture, and the design, execution and analysis of high-throughput cellular assays. Strong quantitative analysis skills required: at a minimum must be able to perform appropriate analysis of large data sets in Excel; familiarity with image analysis/microscopy software a plus. Must be highly organized, meticulous, and adept at professional interactions with a wide variety of investigators on a variety of projects. Must be U.S. citizen or permanent resident. Automated Microscopy The successful candidate will work closely with collaborating investigators on cell culture, assay development, optimization and execution of high-throughput/high-content microscopic assays. Will also coordinate data workflow with screeners and analytic team. Will have primary responsibility for maintenance, quality control and operation of automated screening microscope. Full Time