Biological Studies
Vascular Biology
Vascular permeabilityRole of IKK-ß in Akt activation and vascular permeability. Evans blue dye was injected into a transgenic (TG) and control mouse (CM). Images were taken at 1:30 and 10:00 minutes after injection. Increased vessel leakage was shown in the TG mouse, due to the deletion of IKK-ß, which reduces activation of Akt, and increases vascular permeability.
Comparison of the adhesion of native blood leukocytes (NL) and C-GSF mobilized peripheral blood leukocytes (ML). An image comparing the adhesion of these cells in the vascular endothelium is shown along with a measurement of the number of adhered cells.
Cancer
Leukemia
Transplantation Immunology
T cell conversionA) Image of regulatory T cells (FOXP3-GFP) that have migrated to the islet graft after adoptive transfer. B) Image of effector T cells (U-DsRed) acquired at the same time as A. C) Co-registered image revealing natural regulatory T cells (green), effector T cells (red), and a converted regulatory T cell (yellow).
Simultaneous, independent detection of GFP+ regulatory T cells (blue) and DSRed+ naïve T cells (red)
Investigation of T cell migration in graft versus host disease (GVHD). Images of transplanted T cells near a hair follicle in a (A) freshly irradiated mouse and (B) mixed Chimera (MC) mouse 5 days after transplantation. T cell counts after 5 and 12 days in the irradiated mouse (C) and MC (D). Images of T cells 5 days after transplantation near postcapillary venules in the freshly irradiated mouse (E) and the MC (F).
Stem Cell Biology
Mesenchymal Stem CellsIn vivo confocal and 2-photon microscopy demonstrating the extravasation of FTVI treated MSCs and subsequent migration to the bone surface in the mouse bone marrow after adoptive transfer. DiD-labeled MSCs (red), primarily line the vessel walls (green) at 1 hr after transfer A), whereas at 24 hrs B), the MSCs show a marked increase in extravasation (arrow). Second Harmonic Generation microscopy of bone (collagen, blue) reveals the close juxtaposition between the cells and the bone after 9 days. The bone image in D) is 10µm above the image in C). Bar=50µm for A-B) and 100µm for C-D).
Combined second harmonic and two-photon microscopy image, showing the bone (blue) and Ikaros GFP cells filling the BM. Bar = 50 µm.
Combined second harmonic and confocal microscopy image, showing a single DiD labeled LSK cells sitting on the endosteal surface of the bone (blue) 24 hours after transplantation.
