Brown, Dennis, PhD

Professor

Director: MGH Program in Membrane Biology

Phone: 617-726-5665
Email: Brown.Dennis@mgh.harvard.edu
Lab: Brown, Dennis
Dennis Brown is a Ph. D. cell biologist who specializes in the use of state-of-the art fluorescence imaging and electron microscopy techniques to follow and dissect physiologically-relevant membrane protein trafficking events in epithelial and non-epithelial cells. He serves as the Associate Director of the CSB and the Director of the MGH Program in Membrane Biology (PMB) which uses use state of the art technologies in the pursuit of important biological questions at the system level. He became Professor of Medicine at Harvard in 2001 and has been at MGH since 1985.

Publications

  • Bouley R, Yui N, Terlouw A, Cheung PW, Brown D Chlorpromazine Induces Basolateral Aquaporin-2 Accumulation via F-Actin Depolymerization and Blockade of Endocytosis in Renal Epithelial Cells. Cells. 2020;9(4):ePub - PMID: 32340337 - PMCID: PMC7226349 - DOI: 10.3390/cells9041057

  • Battistone MA, Mendelsohn AC, Spallanzani RG, Allegretti AS, Liberman RN, Sesma J, Kalim S, Wall SM, Bonventre JV, Lazarowski ER, Brown D, Breton S Pro-inflammatory P2Y14 receptor inhibition protects against ischemic acute kidney injury in mice. J Clin Invest. 2020;130(7):3734-3749 - PMID: 32287042 - DOI: 10.1172/JCI134791

  • Cheung PW, Bouley R, Brown D Targeting the Trafficking of Kidney Water Channels for Therapeutic Benefit. Annu Rev Pharmacol Toxicol. 2020;60:175-194 - PMID: 31561739 - DOI: 10.1146/annurev-pharmtox-010919-023654

  • Hoyer FF*, Naxerova K*, Schloss MJ, Hulsmans M, Nair AV, Dutta P, Calcagno DM, Herisson F, Anzai A, Sun Y, Wojtkiewicz G, Rohde D, Frodermann V, Vandoorne K, Courties G, Iwamoto Y, Garris CS, Williams DL, Breton S, Brown D, Whalen M, Libby P, Pittet MJ, King KR, Weissleder R, Swirski FK, Nahrendorf M Tissue-Specific Macrophage Responses to Remote Injury Impact the Outcome of Subsequent Local Immune Challenge. Immunity. 2019;51(5):899-914.e7 - PMID: 31732166 - PMCID: PMC6892583 - DOI: 10.1016/j.immuni.2019.10.010

  • Battistone MA, Spallanzani RG, Mendelsohn AC, Capen D, Nair AV, Brown D, Breton S Novel role of proton-secreting epithelial cells in sperm maturation and mucosal immunity. J Cell Sci. 2019;:ePub - PMID: 31636115 - PMCID: PMC7003979 - DOI: 10.1242/jcs.233239

  • Nair AV, Yanhong W, Paunescu TG, Bouley R, Brown D Sex-dependent differences in water homeostasis in wild-type and V-ATPase B1-subunit deficient mice. PLoS ONE. 2019;14(8):e0219940 - PMID: 31386675 - PMCID: PMC6684071 - DOI: 10.1371/journal.pone.0219940

  • Ferder IC, Fung L, Ohguchi Y, Zhang X, Lassen KG, Capen D, Brown D, Xavier RJ, Wang N Meiotic gatekeeper STRA8 suppresses autophagy by repressing Nr1d1 expression during spermatogenesis in mice. PLoS Genet. 2019;15(5):e1008084 - PMID: 31059511 - PMCID: PMC6502318 - DOI: 10.1371/journal.pgen.1008084

  • Battistone MA, Merkulova M, Park YJ, Peralta MA, Gombar F, Brown D, Breton S Unraveling purinergic regulation in the epididymis: Activation of V-ATPase-dependent acidification by luminal ATP and adenosine. J Physiol. 2019;597(7):1957-1973 - PMID: 30746715 - PMCID: PMC6441927 - DOI: 10.1113/JP277565

  • Cheung PW, Terlouw A, Janssen SA, Brown D, Bouley R Inhibition of non-receptor tyrosine kinase Src induces phosphoserine 256-independent aquaporin-2 membrane accumulation. J Physiol. 2019;597(6):1627-1642 - PMID: 30488437 - PMCID: PMC6418769 - DOI: 10.1113/JP277024

  • Sands JM, Hay M, Brown D APS: Moving Forward to Aid Our Membership. Physiology (Bethesda). 2018;33(6):370-371 - PMID: 30303777 - DOI: 10.1152/physiol.00039.2018

  • Breton S, Brown D Novel Proinflammatory Function of Renal Intercalated Cells. Ann Nutr Metab. 2018;72 Suppl 2:11-16 - PMID: 29925073 - DOI: 10.1159/000488303

  • Chen L, Lee JW, Chou CL, Nair AV, Battistone MA, Păunescu TG, Merkulova M, Breton S, Verlander JW, Wall SM, Brown D, Burg MB, Knepper MA Reply to Edemir: Physiological regulation and single-cell RNA sequencing. Proc Natl Acad Sci U S A. 2018;115(3):E351-E352 - PMID: 29311339 - PMCID: PMC5777009 - DOI: 10.1073/pnas.1720333115

  • Bourgeois S, Bounoure L, Mouro-Chanteloup I, Colin Y, Brown D, Wagner CA The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells. Kidney Int. 2018;93(2):390-402 - PMID: 29054531 - PMCID: PMC6166241 - DOI: 10.1016/j.kint.2017.07.027

  • Chen L, Lee JW, Chou CL, Nair AV, Battistone MA, Păunescu TG, Merkulova M, Breton S, Verlander JW, Wall SM, Brown D, Burg MB, Knepper MA Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq. Proc Natl Acad Sci U S A. 2017;114(46):E9989-E9998 - PMID: 29089413 - PMCID: PMC5699061 - DOI: 10.1073/pnas.1710964114

  • Brown D, Sands JM, Reckelhoff JF APS: The Times They Are A-Changin'. Physiology (Bethesda). 2017;32(6):400-401 - PMID: 28978629 - DOI: 10.1152/physiol.00025.2017

  • Nigwekar SU, Weiser JM, Kalim S, Xu D, Wibecan JL, Dougherty SM, Mercier-Lafond L, Corapi KM, Eneanya ND, Holbrook EH, Brown D, Thadhani RI, Păunescu TG Characterization and Correction of Olfactory Deficits in Kidney Disease. J Am Soc Nephrol. 2017;28(11):3395-3403 - PMID: 28775001 - PMCID: PMC5661283 - DOI: 10.1681/ASN.2016121308

  • Li W, Jin WW, Tsuji K, Chen Y, Nomura N, Su L, Yui N, Arthur J, Cotecchia S, Păunescu TG, Brown D, Lu HAJ Direct interaction of ezrin and AQP2 and its role in AQP2 trafficking. J Cell Sci. 2017;130(17):2914-2925 - PMID: 28754689 - PMCID: PMC5612225 - DOI: 10.1242/jcs.204842

  • Brown D The Discovery of Water Channels (Aquaporins). Ann Nutr Metab. 2017;70 Suppl 1:37-42 - PMID: 28614812 - DOI: 10.1159/000463061

  • Behzadi S, Serpooshan V, Tao W, Hamaly MA, Alkawareek MY, Dreaden EC, Brown D, Alkilany AM, Farokhzad OC, Mahmoudi M Cellular uptake of nanoparticles: journey inside the cell. Chem Soc Rev. 2017;46(14):4218-4244 - PMID: 28585944 - PMCID: PMC5593313 - DOI: 10.1039/c6cs00636a

  • Hulsmans M, Clauss S, Xiao L, Aguirre AD, King KR, Hanley A, Hucker WJ, Wülfers EM, Seemann G, Courties G, Iwamoto Y, Sun Y, Savol AJ, Sager HB, Lavine KJ, Fishbein GA, Capen DE, Da Silva N, Miquerol L, Wakimoto H, Seidman CE, Seidman JG, Sadreyev RI, Naxerova K, Mitchell RN, Brown D, Libby P, Weissleder R, Swirski FK, Kohl P, Vinegoni C, Milan DJ, Ellinor PT, Nahrendorf M Macrophages Facilitate Electrical Conduction in the Heart. Cell. 2017;169:510-522 - PMID: 28431249 - PMCID: PMC5474950 - DOI: 10.1016/j.cell.2017.03.050

  • Cheung PW, Ueberdiek L, Day J, Bouley R, Brown D Protein phosphatase 2C (PP2C) is responsible for VP-induced dephosphorylation of AQP2 serine 261. Am J Physiol Renal Physiol. 2017;:ajprenal.00004.2017 - PMID: 28381458 - PMCID: PMC5582913 - DOI: 10.1152/ajprenal.00004.2017

  • Christensen HL, Păunescu TG, Matchkov V, Barbuskaite D, Brown D, Damkier HH, Praetorius J The V-ATPase is expressed in the choroid plexus and mediates cAMP-induced intracellular pH alterations. Physiol Rep. 2017;5(1):ePub - PMID: 28053225 - PMCID: PMC5256157 - DOI: 10.14814/phy2.13072

  • Chen YC, Backus KM, Merkulova M, Yang C, Brown D, Cravatt BF, Zhang C Covalent Modulators of the Vacuolar ATPase. J Am Chem Soc. 2017;139(2):639-642 - PMID: 28010062 - PMCID: PMC5274637 - DOI: 10.1021/jacs.6b12511

  • Mumtaz R, Trepiccione F, Hennings JC, Huebner AK, Serbin B, Picard N, Ullah AK, Păunescu TG, Capen DE, Lashhab RM, Mouro-Chanteloup I, Alper SL, Wagner CA, Cordat E, Brown D, Eladari D, Hübner CA Intercalated Cell Depletion and Vacuolar H+-ATPase Mistargeting in an AE1 R607H Knockin Model. J Am Soc Nephrol. 2017;28(5):1507-1520 - PMID: 27932475 - PMCID: PMC5407715 - DOI: 10.1681/ASN.2016020169

  • Cheung PW, Nomura N, Nair AV, Pathomthongtaweechai N, Ueberdiek L, Lu HA, Brown D, Bouley R EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol. 2016;27(10):3105-3116 - PMID: 27694161 - PMCID: PMC5042667 - DOI: 10.1681/ASN.2015080903

  • Mamuya FA, Cano-Peñalver JL, Li WU, Rodriguez-Puyol D, Rodríguez-Puyol M, Brown D, de Frutos S, Lu HJ ILK and Cytoskeletal Architecture: An Important Determinant of AQP2 Recycling and Subsequent Entry into the Exocytotic Pathway. Am J Physiol Renal Physiol. 2016;:ajprenal.00336.2016 - PMID: 27760768 - PMCID: PMC5210194 - DOI: 10.1152/ajprenal.00336.2016

  • Reckelhoff JF, Brown D, Molina PE APS Takes a Look in the Mirror. Physiology (Bethesda). 2016;31(6):384-385 - PMID: 27708041 - DOI: 10.1152/physiol.00023.2016

  • Cotter K, Liberman R, Sun-Wada G, Wada Y, Sgroi D, Naber S, Brown D, Breton S, Forgac M The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer. Oncotarget. 2016;7(29):46142-46157 - PMID: 27323815 - PMCID: PMC5216787 - DOI: 10.18632/oncotarget.10063

  • Trepiccione F, Gerber SD, Grahammer F, López-Cayuqueo KI, Baudrie V, Păunescu TG, Capen DE, Picard N, Alexander RT, Huber TB, Chambrey R, Brown D, Houillier P, Eladari D, Simons M Renal Atp6ap2/(Pro)renin Receptor Is Required for Normal Vacuolar H+-ATPase Function but Not for the Renin-Angiotensin System. J Am Soc Nephrol. 2016;27(11):3320-3330 - PMID: 27044666 - PMCID: PMC5084887 - DOI: 10.1681/ASN.2015080915

  • Cheung PW, Nomura N, Nair AV, Pathomthongtaweechai N, Ueberdiek L, Jenny Lu HA, Brown D, Bouley R EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol. 2016;:ePub - PMID: 26961350 - DOI: 10.1681/ASN.2015080903

  • Roy J*, Kim B*, Hill E, Visconti P, Krapf D, Vinegoni C, Weissleder R, Brown D, Breton S Tyrosine kinase-mediated axial motility of basal cells revealed by intravital imaging. Nat Commun. 2016;7:10666 - PMID: 26868824 - PMCID: PMC4754344 - DOI: 10.1038/ncomms10666

  • Inoue Y, Yu YM, Kurihara T, Vasilyev A, Ibrahim A, Oklu R, Zhao G, Nair AV, Brown D, Fischman AJ, Tompkins RG, Irimia D Kidney and Liver Injuries After Major Burns in Rats Are Prevented by Resolvin D2. Crit Care Med. 2016;44(5):e241-52 - PMID: 26509319 - PMCID: PMC4833510 - DOI: 10.1097/CCM.0000000000001397

  • Merkulova M, Păunescu TG, Azroyan A, Marshansky V, Breton S, Brown D Mapping the H(+) (V)-ATPase interactome: identification of proteins involved in trafficking, folding, assembly and phosphorylation. Sci Rep. 2015;5:14827 - PMID: 26442671 - PMCID: PMC4595830 - DOI: 10.1038/srep14827

  • Arthur J, Huang J, Nomura N, Jin WW, Li W, Cheng X, Brown D, Lu HJ Characterization of the putative phosphorylation sites of the AQP2 c-terminus and their role in AQP2 trafficking in LLC-PK1 cells. Am J Physiol Renal Physiol. 2015;:ajprenal.00152.2015 - PMID: 26290367 - PMCID: PMC4609919 - DOI: 10.1152/ajprenal.00152.2015

  • Dutta P, Sager HB, Stengel KR, Naxerova K, Courties G, Saez B, Silberstein L, Heidt T, Sebas M, Sun Y, Wojtkiewicz G, Feruglio PF, King K, Baker JN, van der Laan AM, Borodovsky A, Fitzgerald K, Hulsmans M, Hoyer F, Iwamoto Y, Vinegoni C, Brown D, Di Carli M, Libby P, Hiebert SW, Scadden DT, Swirski FK, Weissleder R, Nahrendorf M Myocardial Infarction Activates CCR2(+) Hematopoietic Stem and Progenitor Cells. Cell Stem Cell. 2015;16 (5):477-487 - PMID: 25957903 - PMCID: PMC4426344 - DOI: 10.1016/j.stem.2015.04.008

  • Azroyan A, Cortez-Retamozo V, Bouley R, Liberman R, Ruan YC, Kiselev E, Jacobson KA, Pittet MJ, Brown D, Breton S Renal Intercalated Cells Sense and Mediate Inflammation via the P2Y14 Receptor. PLoS ONE. 2015;10(3):e0121419 - PMID: 25799465 - PMCID: PMC4370445 - DOI: 10.1371/journal.pone.0121419

  • Nair AV, Keliher EJ, Core AB, Brown D, Weissleder R Characterizing the Interactions of Organic Nanoparticles with Renal Epithelial Cells in Vivo. ACS Nano. 2015;9(4):3641-53 - PMID: 25790730 - PMCID: PMC4414893 - DOI: 10.1021/acsnano.5b00428

  • Herak-Kramberger CM, Breljak D, Ljubojević M, Matokanovic M, Lovric M, Rogic D, Brzica H, Vrhovac I, Karaica D, Micek V, Ivkovic Dupor J, Brown D, Sabolic I Sex-dependent expression of water channel AQP1 along the rat nephron. Am J Physiol Renal Physiol. 2015;:ajprenal.00368.2014 - PMID: 25656365 - PMCID: PMC4398837 - DOI: 10.1152/ajprenal.00368.2014

  • Ruan YC, Wang Y, Da Silva N, Kim B, Diao RY, Hill E, Brown D, Chan HC, Breton S CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation via the ZONAB pathway. J Cell Sci. 2014;127(Pt 20):4396-408 - PMID: 25107366 - PMCID: PMC4197086 - DOI: 10.1242/jcs.148098

  • Păunescu TG, Shum WW, Huynh C, Lechner L, Goetze B, Brown D, Breton S High-resolution helium ion microscopy of epididymal epithelial cells and their interaction with spermatozoa. Mol Hum Reprod. 2014;20(10):929-37 - PMID: 25015675 - PMCID: PMC4172170 - DOI: 10.1093/molehr/gau052

  • Nomura N, Nunes P, Bouley R, Nair AV, Shaw S, Ueda E, Pathomthongtaweechai N, Lu HA, Brown D High throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concentration. Am J Physiol Cell Physiol. 2014;307(7):C597-605 - PMID: 24944200 - PMCID: PMC4187055 - DOI: 10.1152/ajpcell.00154.2014

  • Miranda KC, Bond DT, Levin JZ, Adiconis X, Sivachenko A, Russ C, Brown D, Nusbaum C, Russo LM Massively parallel sequencing of human urinary exosome/microvesicle RNA reveals a predominance of non-coding RNA. PLoS ONE. 2014;9(5):e96094 - PMID: 24816817 - PMCID: PMC4015934 - DOI: 10.1371/journal.pone.0096094

  • Little MH, Brown D, Humphreys BD, McMahon AP, Miner JH, Sands JM, Weisz OA, Mullins C, Hoshizaki D, Defining Kidney Biology to Understand Renal Disease. Clin J Am Soc Nephrol. 2014;9(4):809-11 - PMID: 24370769 - PMCID: PMC3974369 - DOI: 10.2215/CJN.10851013

  • Paunescu TG, Lu HA, Russo LM, Pastor-Soler NM, McKee M, McLaughlin MM, Bartlett BE, Breton S, Brown D Vasopressin induces apical expression of caveolin in rat kidney collecting duct principal cells. Am J Physiol Renal Physiol. 2013;305(12):F1783-F1795 - PMID: 24133120 - PMCID: PMC3882446 - DOI: 10.1152/ajprenal.00622.2012

  • Breton S, Brown D Regulation of Luminal Acidification by the V-ATPase. Physiology (Bethesda). 2013;28(5):318-29 - PMID: 23997191 - PMCID: PMC3768094 - DOI: 10.1152/physiol.00007.2013

  • Shum W, Hill E, Brown D, Breton S Plasticity of basal cells during postnatal development in the rat epididymis. Reproduction. 2013;146(5):455-469 - PMID: 23960170 - PMCID: PMC4106237 - DOI: 10.1530/REP-12-0510

  • Feinstein TN, Yui N, Webber MJ, Wehbi VL, Stevenson HP, King JD, Hallows KR, Brown D, Bouley R, Vilardaga JP Noncanonical control of vasopressin receptor type 2 signaling by retromer and arrestin. J Biol Chem. 2013;288(39):27849-60 - PMID: 23935101 - PMCID: PMC3784700 - DOI: 10.1074/jbc.M112.445098

  • Roy JW, Hill E, Ruan YC, Vedovelli L, Paunescu T, Brown D, Breton S Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis. Am J Physiol Cell Physiol. 2013;305(4):C436-46 - PMID: 23761626 - PMCID: PMC3891217 - DOI: 10.1152/ajpcell.00410.2012

  • Brown D, Jenny Lu HA Aquaporin-2 Inhibitors: Fishing in the Chemical Pool. J Am Soc Nephrol. 2013;24(5):685-6 - PMID: 23599384 - DOI: 10.1681/ASN.2013030243

  • Rice WL, Van Hoek AN, Păunescu TG, Huynh C, Goetze B, Singh B, Scipioni L, Stern LA, Brown D High resolution helium ion scanning microscopy of the rat kidney. PLoS ONE. 2013;8(3):e57051 - PMID: 23505418 - PMCID: PMC3591388 - DOI: 10.1371/journal.pone.0057051

  • Nunes P, Ernandez T, Roth I, Qiao X, Strebel D, Bouley R, Charollais A, Ramadori P, Foti M, Meda P, Féraille E, Brown D, Hasler U Hypertonic stress promotes autophagy and microtubule-dependent autophagosomal clusters. Autophagy. 2013;9(4):550-67 - PMID: 23380587 - PMCID: PMC3627670 - DOI: 10.4161/auto.23662

  • Vedovelli L, Rothermel JT, Finberg KE, Wagner CA, Azroyan A, Hill E, Breton S, Brown D, Paunescu TG Altered V-ATPase expression in renal intercalated cells isolated from B1-subunit deficient mice by fluorescence activated cell sorting. Am J Physiol Renal Physiol. 2013;304(5):F522-32 - PMID: 23269648 - PMCID: PMC3602708 - DOI: 10.1152/ajprenal.00394.2012

  • Bouley R, Nunes P, Andriopoulos B, McLaughlin MM, Webber MJ, Lin HY, Babitt JL, Gardella TJ, Ausiello DA, Brown D Heterologous downregulation of vasopressin type 2 receptor is induced by transferrin. Am J Physiol Renal Physiol. 2013;304(5):F553-64 - PMID: 23235478 - PMCID: PMC3602717 - DOI: 10.1152/ajprenal.00438.2011

  • Yui N, Lu HA, Chen Y, Nomura N, Bouley R, Brown D Basolateral targeting and microtubule dependent transcytosis of the aquaporin-2 water channel. Am J Physiol Cell Physiol. 2013;304(1):C38-48 - PMID: 23015545 - PMCID: PMC3543574 - DOI: 10.1152/ajpcell.00109.2012

  • Yui N, Lu HJ, Bouley R, Brown D AQP2 is necessary for vasopressin- and forskolin-mediated filamentous actin depolymerization in renal epithelial cells. Biol Open. 2012;1(2):101-8 - PMID: 23213402 - PMCID: PMC3507199 - DOI: 10.1242/bio.2011042

  • Păunescu TG, Rodriguez S, Benz E, McKee M, Tyszkowski R, Albers MW, Brown D Loss of the V-ATPase B1 Subunit Isoform Expressed in Non-Neuronal Cells of the Mouse Olfactory Epithelium Impairs Olfactory Function. PLoS ONE. 2012;7(9):e45395 - PMID: 23028982 - PMCID: PMC3447883 - DOI: 10.1371/journal.pone.0045395

  • Hennings JC, Picard N, Huebner AK, Stauber T, Maier H, Brown D, Jentsch TJ, Vargas-Poussou R, Eladari D, Hübner CA A mouse model for distal renal tubular acidosis reveals a previously unrecognized role of the V-ATPase a4 subunit in the proximal tubule. EMBO Mol Med. 2012;4(10):1057-71 - PMID: 22933323 - PMCID: PMC3491836 - DOI: 10.1002/emmm.201201527

  • Chen Y, Rice W, Gu Z, Li J, Huang J, Brenner MB, Van Hoek A, Xiong J, Gundersen GG, Norman JC, Hsu VW, Fenton RA, Brown D, Lu HA Aquaporin 2 Promotes Cell Migration and Epithelial Morphogenesis. J Am Soc Nephrol. 2012;23(9):1506-17 - PMID: 22859853 - PMCID: PMC3431417 - DOI: 10.1681/ASN.2012010079

  • Fan X, Li Q, Pisarek-Horowitz A, Rasouly HM, Wang X, Bonegio RG, Wang H, McLaughlin M, Mangos S, Kalluri R, Holzman LB, Drummond IA, Brown D, Salant DJ, Lu W Inhibitory Effects of Robo2 on Nephrin: A Crosstalk between Positive and Negative Signals Regulating Podocyte Structure. Cell Rep. 2012;2(1):52-61 - PMID: 22840396 - PMCID: PMC3627357 - DOI: 10.1016/j.celrep.2012.06.002

  • Staropoli JF, Haliw L, Biswas S, Garrett L, Hölter SM, Becker L, Skosyrski S, Da Silva-Buttkus P, Calzada-Wack J, Neff F, Rathkolb B, Rozman J, Schrewe A, Adler T, Puk O, Sun M, Favor J, Racz I, Bekeredjian R, Busch DH, Graw J, Klingenspor M, Klopstock T, Wolf E, Wurst W, Zimmer A, Lopez E, Harati H, Hill E, Krause DS, Guide J, Dragileva E, Gale E, Wheeler VC, Boustany RM, Brown DE, Breton S, Ruether K, Gailus-Durner V, Fuchs H, de Angelis MH, Cotman SL Large-Scale Phenotyping of an Accurate Genetic Mouse Model of JNCL Identifies Novel Early Pathology Outside the Central Nervous System. PLoS ONE. 2012;7(6):e38310 - PMID: 22701626 - PMCID: PMC3368842 - DOI: 10.1371/journal.pone.0038310

  • Brown D, Bouley R, Paunescu TG, Breton S, Lu HA New insights into the dynamic regulation of water and acid/base balance by renal epithelial cells. Am J Physiol Cell Physiol. 2012;302(10):C1421-33 - PMID: 22460710 - PMCID: PMC3362000 - DOI: 10.1152/ajpcell.00085.2012

  • Rice WL, Zhang Y, Chen Y, Matsuzaki T, Brown D, Lu HA Differential, Phosphorylation Dependent Trafficking of AQP2 in LLC-PK1 Cells. PLoS ONE. 2012;7(2):e32843 - PMID: 22403603 - PMCID: PMC3293519 - DOI: 10.1371/journal.pone.0032843

  • Brown D, Wagner CA Molecular Mechanisms of Acid-Base Sensing by the Kidney. J Am Soc Nephrol. 2012;23(5):774-80 - PMID: 22362904 - PMCID: PMC3338302 - DOI: 10.1681/ASN.2012010029

  • Wagner CA, Mohebbi N, Uhlig U, Giebisch GH, Breton S, Brown D, Geibel JP Angiotensin II Stimulates H-ATPase Activity in Intercalated Cells from Isolated Mouse Connecting Tubules and Cortical Collecting Ducts. Cell Physiol Biochem. 2011;28(3):513-20 - PMID: 22116365 - PMCID: PMC3709473 - DOI: 10.1159/000335112

  • Winter C, Kampik NB, Vedovelli L, Rothenberger F, Paunescu TG, Stehberger PA, Brown D, John H, Wagner CA Aldosterone stimulates vacuolar H+-ATPase activity in renal acid-secretory intercalated cells mainly via a protein kinase C dependent pathway. Am J Physiol Cell Physiol. 2011;301(5):C1251-61 - PMID: 21832245 - PMCID: PMC3213922 - DOI: 10.1152/ajpcell.00076.2011

  • Chen S, Webber MJ, Vilardaga JP, Khatri A, Brown D, Ausiello DA, Lin HY, Bouley R Visualizing Microtubule-Dependent Vasopressin Type 2 Receptor Trafficking Using a New High-Affinity Fluorescent Vasopressin Ligand. Endocrinology. 2011;152(10):3893-904 - PMID: 21828182 - PMCID: PMC3176653 - DOI: 10.1210/en.2011-1049

  • Li W, Zhang Y, Bouley R, Chen Y, Matsuzaki T, Nunes P, Hasler U, Brown D, Lu HA Simvastatin Enhances Aquaporin 2 Surface Expression and Urinary Concentration in Vasopressin Deficient Brattleboro Rats through Modulation of Rho GTPase. Am J Physiol Renal Physiol. 2011;301(2):F309-18 - PMID: 21511701 - PMCID: PMC3154588 - DOI: 10.1152/ajprenal.00001.2011

  • Shum WW, Da Silva N, Belleannee C, McKee M, Brown D, Breton S Regulation of V-ATPase recycling via a RhoA and ROCKII dependent pathway in epididymal clear cells. Am J Physiol Cell Physiol. 2011;301(1):C31-43 - PMID: 21411727 - PMCID: PMC3129830 - DOI: 10.1152/ajpcell.00198.2010

  • Merkulova M, Hurtado-Lorenzo A, Hosokawa H, Zhuang Z, Brown D, Ausiello DA, Marshansky V Aldolase directly interacts with ARNO and modulates cell morphology and acidic vesicle distribution. Am J Physiol Cell Physiol. 2011;300(6):C1442-55 - PMID: 21307348 - PMCID: PMC3118619 - DOI: 10.1152/ajpcell.00076.2010

  • Da Silva N, Cortez-Retamozo V, Reinecker HC, Wildgruber M, Hill E, Brown D, Swirski F, Pittet M, Breton S A dense network of dendritic cells populates the murine epididymis. Reproduction. 2011;141(5):653-63 - PMID: 21310816 - PMCID: PMC3657760 - DOI: 10.1530/REP-10-0493

  • Bouley R, Lu HA, Nunes P, Da Silva N, McLaughlin M, Chen Y, Brown D Calcitonin Has a Vasopressin-like Effect on Aquaporin-2 Trafficking and Urinary Concentration. J Am Soc Nephrol. 2011;22(1):59-72 - PMID: 21071524 - PMCID: PMC3014035 - DOI: 10.1681/ASN.2009121267

  • Zhuang Z, Marshansky V, Breton S, Brown D Is caveolin involved in normal proximal tubule (PT) function? Presence in model PT systems but absence in situ. Am J Physiol Renal Physiol. 2011;300(1):F199-206 - PMID: 20980408 - PMCID: PMC3023221 - DOI: 10.1152/ajprenal.00513.2010

  • Gao X, Eladari D, Leviel F, Tew BY, Miro-Julia Deletion of hensin/DMBT1 blocks conversion of {beta}- to {alpha}-intercalated cells and induces distal renal tubular acidosis. Proc Natl Acad Sci U S A. 2010;107(50):21872-7 - PMID: 21098262 - PMCID: PMC3003085 - DOI: 10.1073/pnas.1010364107

  • Schreiner R, Frindt G, Diaz F, Carvajal-Gonzalez JM, Perez Bay AE, Palmer LG, Marshansky V, Brown D, Philp NJ, Rodriguez-Boulan E The absence of a clathrin adapter confers unique polarity essential to proximal tubule function. Kidney Int. 2010;78(4):382-8 - PMID: 20531453 - PMCID: PMC3684398 - DOI: 10.1038/ki.2010.166

  • Miranda KC, Bond DT, McKee M, Skog J, Paunescu TG, Da Silva N, Brown D, Russo LM Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease. Kidney Int. 2010;78(2):191-9 - PMID: 20428099 - PMCID: PMC4451567 - DOI: 10.1038/ki.2010.106

  • Miranda KC, Karet FE, Brown D An Extended Nomenclature for Mammalian V-ATPase Subunit Genes and Splice Variants. PLoS ONE. 2010;5(3):e9531 - PMID: 20224822 - PMCID: PMC2835735 - DOI: 10.1371/journal.pone.0009531

  • Da Silva N, Pisitkun T, Belleannee C, Miller LR, Nelson RD, Knepper MA, Brown D, Breton S Proteomic analysis of V-ATPase-rich cells harvested from the kidney and epididymis by fluorescence activated cell sorting. Am J Physiol Cell Physiol. 2010;298(6):C1326-42 - PMID: 20181927 - PMCID: PMC2889637 - DOI: 10.1152/ajpcell.00552.2009

  • Xia Y, Babitt JL, Bouley R, Zhang Y, Silva ND, Chen S, Zhuang Z, Samad TA, Brenner GJ, Anderson JL, Hong CC, Schneyer AL, Brown D, Lin HY Dragon Enhances BMP Signaling and Increases Transepithelial Resistance in Kidney Epithelial Cells. J Am Soc Nephrol. 2010;21(4):666-77 - PMID: 20167703 - PMCID: PMC2844302 - DOI: 10.1681/ASN.2009050511

  • Belleannee C, Da Silva N, Shum WW, Brown D, Breton S Role of purinergic signaling pathways in V-ATPase recruitment to the apical membrane of acidifying epididymal clear cells. Am J Physiol Cell Physiol. 2010;298(4):C817-30 - PMID: 20071692 - PMCID: PMC2853219 - DOI: 10.1152/ajpcell.00460.2009

  • Paunescu TG, Ljubojevic M, Russo LM, Winter C, McLaughlin MM, Wagner CA, Breton S, Brown D Cyclic AMP stimulates apical V-ATPase accumulation, microvillar elongation and proton extrusion in kidney collecting duct A-intercalated cells. Am J Physiol Renal Physiol. 2010;298(3):F643-54 - PMID: 20053793 - PMCID: PMC2838605 - DOI: 10.1152/ajprenal.00584.2009

  • Pastor-Soler NM, Fisher JS, Sharpe R, Hill E, Van Hoek A, Brown D, Breton S Aquaporin 9 expression in the developing rat epididymis is modulated by steroid hormones. Reproduction. 2010;139(3):613-21 - PMID: 19948840 - PMCID: PMC3773695 - DOI: 10.1530/REP-09-0284

  • Bouley R, Palomino Z, Tang SS, Nunes P, Kobori H, Lu HA, Shum WW, Sabolic I, Brown D, Ingelfinger JR, Jung FF Angiotensin II and Hypertonicity Modulate Proximal Tubular Aquaporin 1 (AQP1) Expression. Am J Physiol Renal Physiol. 2009;297(6):F1575-86 - PMID: 19776169 - PMCID: PMC2801332 - DOI: 10.1152/ajprenal.90762.2008

  • Shum WW, Da Silva N, Brown D, Breton S Regulation of luminal acidification in the male reproductive tract via cell-cell crosstalk. J Exp Biol. 2009;212(Pt 11):1753-61 - PMID: 19448084 - PMCID: PMC2683015 - DOI: 10.1242/jeb.027284

  • Brown D, Paunescu TG, Breton S, Marshansky V Regulation of the V-ATPase in kidney epithelial cells: dual role in acid-base homeostasis and vesicle trafficking. J Exp Biol. 2009;212(Pt 11):1762-72 - PMID: 19448085 - PMCID: PMC2683016 - DOI: 10.1242/jeb.028803

  • Van Hoek AN, Bouley R, Lu YX, Silberstein CM, Brown D, Wax MB, Patil RV Vasopressin-induced differential stimulation of AQP4 splice variants regulates the in-membrane assembly of orthogonal arrays. Am J Physiol Renal Physiol. 2009;296(6):F1396-404 - PMID: 19297454 - PMCID: PMC2692439 - DOI: 10.1152/ajprenal.00018.2009

  • Brown D, Breton S, Ausiello DA, Marshansky V Sensing, Signaling and Sorting Events in Kidney Epithelial Cell Physiology. Traffic. 2009;10(3):275-84 - PMID: 19170982 - PMCID: PMC2896909 - DOI: 10.1111/j.1600-0854.2008.00867.x

  • Russo LM, Sandoval RM, Campos SB, Molitoris BA, Comper WD, Brown D Impaired Tubular Uptake Explains Albuminuria in Early Diabetic Nephropathy. J Am Soc Nephrol. 2009;20(3):489-94 - PMID: 19118149 - PMCID: PMC2653682 - DOI: 10.1681/ASN.2008050503

  • Miller RL, Lucero OM, Riemondy KA, Baumgartner BK, Brown D, Breton S, Nelson RD The V-ATPase B1-subunit promoter drives expression of Cre recombinase in intercalated cells of the kidney. Kidney Int. 2009;75(4):435-9 - PMID: 19052537 - PMCID: PMC4301582 - DOI: 10.1038/ki.2008.569

  • Belleannee C, Da Silva N, Shum WW, Marsolais M, Laprade R, Brown D, Breton S Segmental Expression of the Bradykinin Type 2 Receptor in Rat Efferent Ducts and Epididymis and Its Role in the Regulation of Aquaporin 9. Biol Reprod. 2009;80(1):134-43 - PMID: 18829705 - PMCID: PMC2804812 - DOI: 10.1095/biolreprod.108.070797

  • Russo LM, Brown D, Lin HY The soluble transforming growth factor-beta receptor: Advantages and applications. Int J Biochem Cell B. 2009;41(3):472-6 - PMID: 18339576 - DOI: 10.1016/j.biocel.2008.01.026

  • Shum WW, Da Silva N, McKee M, Smith PJ, Brown D, Breton S Transepithelial Projections from Basal Cells are Luminal Sensors in Pseudostratified Epithelia. Cell. 2008;135:1108-17 - PMID: 19070580 - PMCID: PMC2646085 - DOI: 10.1016/j.cell.2008.10.020

  • Zalyapin EA, Bouley R, Hasler U, Vilardaga JP, Lin HY, Brown D, Ausiello DA Effects of the renal medullary pH and ionic environment on vasopressin binding and signaling. Kidney Int. 2008;74(12):1557-67 - PMID: 18813286 - PMCID: PMC3730289 - DOI: 10.1038/ki.2008.412

  • Nunes P, Hasler U, McKee M, Lu HA, Bouley R, Brown D A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2 (AQP2). Am J Physiol Cell Physiol. 2008;295(6):C1476-87 - PMID: 18799651 - PMCID: PMC2603565 - DOI: 10.1152/ajpcell.00344.2008

  • Brown D, Hasler U, Nunes P, Bouley R, Lu H Phosphorylation events and the modulation of aquaporin 2 cell surface expression. Curr Opin Nephrol Hypertens. 2008;17(5):491-8 - PMID: 18695390 - PMCID: PMC3774073 - DOI: 10.1097/MNH.0b013e3283094eb1

  • Hasler U, Nunes P, Bouley R, Lu HA, Matsuzaki T, Brown D Acute hypertonicity alters aquaporin-2 trafficking and induces a MAP kinase-dependent accumulation at the plasma membrane of renal epithelial cells. J Biol Chem. 2008;283(39):26643-61 - PMID: 18664568 - PMCID: PMC2546545 - DOI: 10.1074/jbc.M801071200

  • Paunescu TG, Jones AC, Tyszkowski R, Brown D V-ATPase expression in the mouse olfactory epithelium. Am J Physiol Cell Physiol. 2008;295(4):C923-30 - PMID: 18667600 - PMCID: PMC2575830 - DOI: 10.1152/ajpcell.00237.2008

  • Brown D Aquaporins and vasopressin signaling in kidney health and disease: introduction. Semin Nephrol. 2008;28:215-6 - PMID: 18519082 - DOI: 10.1016/j.semnephrol.2008.03.001

  • Bouley R, Hasler U, Lu HA, Nunes P, Brown D Bypassing vasopressin receptor signaling pathways in nephrogenic diabetes insipidus. Semin Nephrol. 2008;28:266-78 - PMID: 18519087 - PMCID: PMC2494582 - DOI: 10.1016/j.semnephrol.2008.03.010

  • Lu HA, Matsuzaki T, Bouley R, Hasler U, Qin Q, Brown D The phosphorylation state of serine 256 is dominant over that of serine 261 in the regulation of AQP2 trafficking in renal epithelial cells. Am J Physiol Renal Physiol. 2008;295(1):F290-4 - PMID: 18434387 - PMCID: PMC2494506 - DOI: 10.1152/ajprenal.00072.2008

  • Pietrement C, Da Silva N, Silberstein C, James M, Marsolais M, Van Hoek A, Brown D, Pastor-Soler N, Ameen N, Laprade R, Ramesh V, Breton S Role of NHERF1, CFTR and cAMP in the regulation of aquaporin 9. J Biol Chem. 2008;283:2986-96 - PMID: 18055461 - DOI: 10.1074/jbc.M704678200

  • Paunescu TG, Da Silva N, Russo LM, McKee M, Lu HA, Breton S, Brown D Association Of Soluble Adenylyl Cyclase (sAC) With The V-ATPase In Renal Epithelial Cells. Am J Physiol Renal Physiol. 2008;294(1):F130-8 - PMID: 17959750 - DOI: 10.1152/ajprenal.00406.2007

  • Breton S, Brown D New insights into the regulation of V-ATPase-dependent proton secretion. Am J Physiol Renal Physiol. 2007;292(1):F1-10 - PMID: 17032935 - DOI: 10.1152/ajprenal.00340.2006

  • Russo LM, Sandoval RM, Brown D, Molitoris BA, Comper WD Response to ''On the origin of albuminuria''. Kidney Int. 2007;72:1409-10 - PMID: 18004312

  • Russo LM, Sandoval RM, Brown D, Molitoris BA, Comper WD Response to ''Albumin concentration in the Bowman's capsule: Multiphoton microscopy vs micropuncture technique''. Kidney Int. 2007;72:1411 - PMID: 18004315

  • Paunescu TG, Russo LM, Da Silva N, Kovacikova J, Mohebbi N, Van Hoek AN, McKee M, Wagner CA, Breton S, Brown D Compensatory membrane expression of the V-ATPase B2 subunit isoform in renal medullary intercalated cells of B1-deficient mice. Am J Physiol Renal Physiol. 2007;293(6):F1915-26 - PMID: 17898041 - DOI: 10.1152/ajprenal.00160.2007

  • Russo LM, Sandoval RM, Brown D, Molitoris BA, Comper WD Controversies in nephrology: Response to ''renal albumin handling, facts, and artifacts''. Kidney Int. 2007;72(10):1195-7 - PMID: 17851467 - DOI: 10.1038/sj.ki.5002528

  • Lu HA, Sun TX, Matsuzaki T, Yi XH, Eswara J, Bouley R, McKee M, Brown D Heat shock protein 70 interacts with aquaporin-2 (AQP2) and regulates its trafficking. J Biol Chem. 2007;282(39):28721-32 - PMID: 17636261 - DOI: 10.1074/jbc.M611101200

  • Da Silva N, Shum WW, El-Annan J, Paunescu TG, McKee M, Smith PJ, Brown D, Breton S Relocalization of the V-ATPase B2 subunit to the apical membrane of epididymal clear cells of mice deficient in the B1 subunit. Am J Physiol Cell Physiol. 2007;293(1):C199-210 - PMID: 17392376 - DOI: 10.1152/ajpcell.00596.2006

  • Russo LM, del Re E, Brown D, Lin HY Evidence for a role of transforming growth factor (TGF)-beta1 in the induction of postglomerular albuminuria in diabetic nephropathy: amelioration by soluble TGF-beta type II receptor. Diabetes. 2007;56(2):380-8 - PMID: 17259382 - DOI: 10.2337/db06-1018

  • Russo LM, Sandoval RM, McKee M, Osicka TM, Collins AB, Brown D, Molitoris BA, Comper WD The normal kidney filters nephrotic levels of albumin retrieved by proximal tubule cells: retrieval is disrupted in nephrotic states. Kidney Int. 2007;71(6):504-13 - PMID: 17228368 - DOI: 10.1038/sj.ki.5002041

  • Yi X, Bouley R, Lin HY, Bechoua S, Sun TX, Del Re E, Shioda T, Raychowdhury MK, Lu HA, Abou-Samra AB, Brown D, Ausiello DA Alix (AIP1) is a vasopressin receptor (V2R)-interacting protein that increases lysosomal degradation of the V2R. Am J Physiol Renal Physiol. 2007;292(5):F1303-13 - PMID: 17287200 - DOI: 10.1152/ajprenal.00441.2005

  • Lisi S, Botta R, Pinchera A, Collins AB, Refetoff S, Arvan P, Bu G, Grasso L, Marshansky V, Bechoua S, Hurtado-Lorenzo A, Marcocci C, Brown D, McCluskey RT, Marinò M Defective thyroglobulin storage in LDL receptor-associated protein-deficient mice. Am J Physiol Cell Physiol. 2006;290(4):C1160-7 - PMID: 16306127 - DOI: 10.1152/ajpcell.00382.2005

  • Da Silva N, Pietrement C, Brown D, Breton S Segmental and cellular expression of aquaporins in the male excurrent duct. Biochim Biophys Acta. 2006;1758(8):1025-33 - PMID: 16935257 - DOI: 10.1016/j.bbamem.2006.06.026

  • Pietrement C, Sun-Wada GH, Silva ND, McKee M, Marshansky V, Brown D, Futai M, Breton S Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis. Biol Reprod. 2006;74(1):185-94 - PMID: 16192400 - DOI: 10.1095/biolreprod.105.043752

  • Da Silva N, Silberstein C, Beaulieu V, Pietrement C, Van Hoek AN, Brown D, Breton S Postnatal expression of aquaporins in epithelial cells of the rat epididymis. Biol Reprod. 2006;74(2):427-38 - PMID: 16221990 - DOI: 10.1095/biolreprod.105.044735

  • Sabolic I, Herak-Kramberger CM, Antolovic R, Breton S, Brown D Loss of basolateral invaginations in proximal tubules of cadmium-intoxicated rats is independent of microtubules and clathrin. Toxicology. 2006;218(2-3):149-63 - PMID: 16288946 - DOI: 10.1016/j.tox.2005.10.009

  • Bouley R, Hawthorn G, Russo L, Lin H, Ausiello D, Brown D Aquaporin 2 (AQP2) and vasopressin type 2 receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in 'endocytosis-resistant' membrane domains after vasopressin treatment. Biol Cell. 2006;98(4):215-32 - PMID: 16563128 - DOI: 10.1042/BC20040054

  • Hurtado-Lorenzo A, Skinner M, El Annan J, Futai M, Sun-Wada GH, Bourgoin S, Casanova J, Wildeman A, Bechoua S, Ausiello DA, Brown D, Marshansky V V-ATPase interacts with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway. Nat Cell Biol. 2006;8(2):124-36 - PMID: 16415858 - DOI: 10.1038/ncb1348

  • Bouley R, Lin HY, Raychowdhury MK, Marshansky V, Brown D, Ausiello DA Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: involvement of a lysosomal degradation pathway. Am J Physiol Cell Physiol. 2005;288(6):C1390-401 - PMID: 15677378 - DOI: 10.1152/ajpcell.00353.2004

  • Miller RL, Zhang P, Smith M, Beaulieu V, Paunescu TG, Brown D, Breton S, Nelson RD V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice. Am J Physiol Cell Physiol. 2005;288(5):C1134-44 - PMID: 15634743 - DOI: 10.1152/ajpcell.00084.2004

  • Bouley R, Hawthorn G, Russo L, Lin HY, Ausiello D, Brown D Aquaporin 2 (AQP2) and vasopressin receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in "endocytosis-resistant" membrane domains after vasopressin treatment. Abdom Imaging. 2005;:ePub - PMID: 16104839

  • Bouley R, Pastor-Soler N, Cohen O, McLaughlin M, Breton S, Brown D Stimulation of AQP2 membrane insertion in renal epithelial cells in vitro and in vivo by the cGMP phosphodiesterase inhibitor sildenafil citrate (Viagra). Am J Physiol Renal Physiol. 2005;288(6):F1103-12 - PMID: 15644488 - DOI: 10.1152/ajprenal.00337.2004

  • Beaulieu V, Da Silva N, Pastor-Soler N, Brown CR, Smith PJ, Brown D, Breton S Modulation of the actin cytoskeleton via gelsolin regulates vacuolar H+-ATPase recycling. J Biol Chem. 2005;280(9):8452-63 - PMID: 15591047 - DOI: 10.1074/jbc.M412750200

  • Finberg KE, Wagner CA, Bailey MA, Paunescu TG, Breton S, Brown D, Giebisch G, Geibel JP, Lifton RP The B1-subunit of the H(+) ATPase is required for maximal urinary acidification. Proc Natl Acad Sci U S A. 2005;102(38):13616-21 - PMID: 16174750 - PMCID: PMC1224669 - DOI: 10.1073/pnas.0506769102

  • Reiser J, Polu KR, Moller CC, Kenlan P, Altintas MM, Wei C, Faul C, Herbert S, Villegas I, Avila-Casado C, McGee M, Sugimoto H, Brown D, Kalluri R, Mundel P, Smith PL, Clapham DE, Pollak MR TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function. Nat Genet. 2005;37(7):739-44 - PMID: 15924139 - PMCID: PMC1360984 - DOI: 10.1038/ng1592

  • Wagner CA, Finberg KE, Breton S, Marshansky V, Brown D, Geibel JP Renal vacuolar H+-ATPase. 2004;84(4):1263-314 - PMID: 15383652 - DOI: 10.1152/physrev.00045.2003

  • El-Annan J, Brown D, Breton S, Bourgoin S, Ausiello DA, Marshansky V Differential expression and targeting of endogenous Arf1 and Arf6 small GTPases in kidney epithelial cells in situ. Am J Physiol Cell Physiol. 2004;286(4):C768-78 - PMID: 14684384 - DOI: 10.1152/ajpcell.00250.2003

  • Paunescu TG, Da Silva N, Marshansky V, McKee M, Breton S, Brown D Expression of the 56-kDa B2 subunit isoform of the vacuolar H(+)-ATPase in proton-secreting cells of the kidney and epididymis. Am J Physiol Cell Physiol. 2004;287(1):C149-62 - PMID: 15013950 - DOI: 10.1152/ajpcell.00464.2003

  • Wagner CA, L A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse. Pflug Arch Eur J Phy. 2003;446(5):623-32 - PMID: 12748863 - DOI: 10.1007/s00424-003-1082-3

  • van Balkom BW, van Raak M, Breton S, Pastor-Soler N, Bouley R, van der Sluijs P, Brown D, Deen PM Hypertonicity is involved in redirecting the aquaporin-2 water channel into the basolateral, instead of the apical, plasma membrane of renal epithelial cells. J Biol Chem. 2003;278(2):1101-7 - PMID: 12374804 - DOI: 10.1074/jbc.M207339200

  • Pastor-Soler N, Beaulieu V, Litvin TN, Da Silva N, Chen Y, Brown D, Buck J, Levin LR, Breton S Bicarbonate-regulated adenylyl cyclase (sAC) is a sensor that regulates pH-dependent V-ATPase recycling. J Biol Chem. 2003;278(49):49523-9 - PMID: 14512417 - PMCID: PMC3652382 - DOI: 10.1074/jbc.M309543200

  • Isnard-Bagnis C, Da Silva N, Beaulieu V, Yu AS, Brown D, Breton S Detection of ClC-3 and ClC-5 in epididymal epithelium: immunofluorescence and RT-PCR after LCM. Am J Physiol Cell Physiol. 2003;284(1):C220-32 - PMID: 12475763 - DOI: 10.1152/ajpcell.00374.2001

  • Marshansky V, Ausiello DA, Brown D Physiological importance of endosomal acidification: potential role in proximal tubulopathies. Curr Opin Nephrol Hypertens. 2002;11(5):527-37 - PMID: 12187318 - DOI: 10.1097/00041552-200209000-00009

  • Zharkikh L, Zhu X, Stricklett PK, Kohan DE, Chipman G, Breton S, Brown D, Nelson RD Renal principal cell-specific expression of green fluorescent protein in transgenic mice. Am J Physiol Renal Physiol. 2002;283(6):F1351-64 - PMID: 12426236 - DOI: 10.1152/ajprenal.0224.2001

  • Pastor-Soler N, Isnard-Bagnis C, Herak-Kramberger C, Sabolic I, Van Hoek A, Brown D, Breton S Expression of aquaporin 9 in the adult rat epididymal epithelium is modulated by androgens. Biol Reprod. 2002;66(6):1716-22 - PMID: 12021052 - DOI: 10.1095/biolreprod66.6.1716

  • Breton S, Inoue T, Knepper MA, Brown D Antigen retrieval reveals widespread basolateral expression of syntaxin 3 in renal epithelia. Am J Physiol Renal Physiol. 2002;282(3):F523-9 - PMID: 11832435 - DOI: 10.1152/ajprenal.00128.2001

  • Tekirian TL, Merriam DE, Marshansky V, Miller J, Crowley AC, Chan H, Ausiello D, Brown D, Buxbaum JD, Xia W, Wasco W Subcellular localization of presenilin 2 endoproteolytic C-terminal fragments. Brain Res Mol Brain Res. 2001;96(1-2):14-20 - PMID: 11731004 - DOI: 10.1016/s0169-328x(01)00250-9

  • Maranda B, Brown D, Bourgoin S, Casanova JE, Vinay P, Ausiello DA, Marshansky V Intra-endosomal pH-sensitive recruitment of the Arf-nucleotide exchange factor ARNO and Arf6 from cytoplasm to proximal tubule endosomes. J Biol Chem. 2001;276(21):18540-50 - PMID: 11278939 - DOI: 10.1074/jbc.M011577200

  • Pastor-Soler N, Bagnis C, Sabolic I, Tyszkowski R, McKee M, Van Hoek A, Breton S, Brown D Aquaporin 9 expression along the male reproductive tract. Biol Reprod. 2001;65(2):384-93 - PMID: 11466204 - DOI: 10.1095/biolreprod65.2.384

  • Herak-Kramberger CM, Breton S, Brown D, Kraus O, Sabolic I Distribution of the vacuolar H+ atpase along the rat and human male reproductive tract. Biol Reprod. 2001;64(6):1699-707 - PMID: 11369598 - DOI: 10.1095/biolreprod64.6.1699

  • Bagnis C, Marshansky V, Breton S, Brown D Remodeling the cellular profile of collecting ducts by chronic carbonic anhydrase inhibition. Am J Physiol Renal Physiol. 2001;280(3):F437-48 - PMID: 11181405 - DOI: 10.1152/ajprenal.2001.280.3.F437

  • Choukroun GJ, Marshansky V, Gustafson CE, McKee M, Hajjar RJ, Rosenzweig A, Brown D, Bonventre JV Cytosolic phospholipase A(2) regulates golgi structure and modulates intracellular trafficking of membrane proteins. J Clin Invest. 2000;106(8):983-93 - PMID: 11032858 - PMCID: PMC314339 - DOI: 10.1172/JCI8914

  • Sullivan BM, Harrison-Lavoie KJ, Marshansky V, Lin HY, Kehrl JH, Ausiello DA, Brown D, Druey KM RGS4 and RGS2 bind coatomer and inhibit COPI association with Golgi membranes and intracellular transport. Mol Biol Cell. 2000;11(9):3155-68 - PMID: 10982407 - PMCID: PMC14982 - DOI: 10.1091/mbc.11.9.3155

  • Brown D, Breton S H(+)V-ATPase-dependent luminal acidification in the kidney collecting duct and the epididymis/vas deferens: vesicle recycling and transcytotic pathways. J Exp Biol. 2000;203(Pt 1):137-45 - PMID: 10600682

  • Brown D, Breton S Sorting proteins to their target membranes. Kidney Int. 2000;57(3):816-24 - PMID: 10720934 - DOI: 10.1046/j.1523-1755.2000.00920.x

  • Stevens AL, Breton S, Gustafson CE, Bouley R, Nelson RD, Kohan DE, Brown D Aquaporin 2 is a vasopressin-independent, constitutive apical membrane protein in rat vas deferens. Am J Physiol Cell Physiol. 2000;278(4):C791-802 - PMID: 10751327 - DOI: 10.1152/ajpcell.2000.278.4.C791

  • Silver RB, Breton S, Brown D Potassium depletion increases proton pump (H(+)-ATPase) activity in intercalated cells of cortical collecting duct. Am J Physiol Renal Physiol. 2000;279(1):F195-202 - PMID: 10894802 - DOI: 10.1152/ajprenal.2000.279.1.F195

  • Herak-Kramberger CM, Sabolić I, Blanusa M, Smith PJ, Brown D, Breton S Cadmium inhibits vacuolar H(+)ATPase-mediated acidification in the rat epididymis. Biol Reprod. 2000;63(2):599-606 - PMID: 10906070 - DOI: 10.1095/biolreprod63.2.599

  • Breton S, Wiederhold T, Marshansky V, Nsumu NN, Ramesh V, Brown D The B1 subunit of the H+ATPase is a PDZ domain-binding protein. Colocalization with NHE-RF in renal B-intercalated cells. J Biol Chem. 2000;275(24):18219-24 - PMID: 10748165 - DOI: 10.1074/jbc.M909857199

  • Breton S, Nsumu NN, Galli T, Sabolic I, Smith PJ, Brown D Tetanus toxin-mediated cleavage of cellubrevin inhibits proton secretion in the male reproductive tract. Am J Physiol Renal Physiol. 2000;278(5):F717-25 - PMID: 10807583 - DOI: 10.1152/ajprenal.2000.278.5.F717

  • Bouley R, Breton S, Sun T, McLaughlin M, Nsumu NN, Lin HY, Ausiello DA, Brown D Nitric oxide and atrial natriuretic factor stimulate cGMP-dependent membrane insertion of aquaporin 2 in renal epithelial cells. J Clin Invest. 2000;106(9):1115-26 - PMID: 11067864 - PMCID: PMC301414 - DOI: 10.1172/JCI9594

  • Shayakul C, Breton S, Brown D, Alper SL Gene therapy of inherited renal tubular disease. Am J Kidney Dis. 1999;34(2):374-9 - PMID: 10430991 - DOI: 10.1016/s0272-6386(99)70372-0

  • Sabolić I, Herak-Kramberger CM, Breton S, Brown D Na/K-ATPase in intercalated cells along the rat nephron revealed by antigen retrieval. J Am Soc Nephrol. 1999;10(5):913-22 - PMID: 10232676

  • Jensen LJ, Schmitt BM, Berger UV, Nsumu NN, Boron WF, Hediger MA, Brown D, Breton S Localization of sodium bicarbonate cotransporter (NBC) protein and messenger ribonucleic acid in rat epididymis. Biol Reprod. 1999;60(3):573-9 - PMID: 10026101 - DOI: 10.1095/biolreprod60.3.573

  • Breton S, Tyszkowski R, Sabolic I, Brown D Postnatal development of H+ ATPase (proton-pump)-rich cells in rat epididymis. Histochem Cell Biol. 1999;111(2):97-105 - PMID: 10090570 - DOI: 10.1007/s004180050339

  • Breton S, Hammar K, Smith PJS, Brown D Proton secretion in the male reproductive tract: involvement of Cl--independent HCO 3 - transport. Am J Physiol Cell Physiol. 1998;275(4):C1134-C1142 - PMID: 29585324 - DOI: 10.1152/ajpcell.1998.275.4.C1134

  • Zuk A, Bonventre JV, Brown D, Matlin KS Polarity, integrin, and extracellular matrix dynamics in the postischemic rat kidney. Am J Physiol Cell Physiol. 1998;275(3):C711-C731 - PMID: 29585718 - DOI: 10.1152/ajpcell.1998.275.3.C711

  • Brown D, Sabolic I, Breton S Polarized expression of membrane proteins in renal epithelial cells: involvement of specialized transport vesicles and intracellular pathways. Adv Nephrol Necker Hosp. 1998;27:297-315 - PMID: 9408453

  • Breton S, Lisanti MP, Tyszkowski R, McLaughlin M, Brown D Basolateral distribution of caveolin-1 in the kidney. Absence from H+-atpase-coated endocytic vesicles in intercalated cells. J Histochem Cytochem. 1998;46(2):205-14 - PMID: 9446827 - DOI: 10.1177/002215549804600209

  • Breton S, Hammar K, Smith PJ, Brown D Proton secretion in the male reproductive tract: involvement of Cl--independent HCO-3 transport. Am J Physiol. 1998;275(4 Pt 1):C1134-42 - PMID: 9755067 - DOI: 10.1152/ajpcell.1998.275.4.C1134

  • Breton S, Brown D Cold-induced microtubule disruption and relocalization of membrane proteins in kidney epithelial cells. J Am Soc Nephrol. 1998;9(2):155-66 - PMID: 9527391

  • Brown D, Lee R, Bonventre JV Redistribution of villin to proximal tubule basolateral membranes after ischemia and reperfusion. Am J Physiol Renal Physiol. 1997;273(6):F1003-F1012 - PMID: 29586118 - DOI: 10.1152/ajprenal.1997.273.6.F1003

  • Kawachi H, Kurihara H, Topham PS, Brown D, Shia MA, Orikasa M, Shimizu F, Salant DJ Slit diaphragm-reactive nephritogenic MAb 5-1-6 alters expression of ZO-1 in rat podocytes. Am J Physiol Renal Physiol. 1997;273(6):F984-F993 - PMID: 29586119 - DOI: 10.1152/ajprenal.1997.273.6.F984

  • Shayakul C, Kanai Y, Lee WS, Brown D, Rothstein JD, Hediger MA Localization of the high-affinity glutamate transporter EAAC1 in rat kidney. Am J Physiol Renal Physiol. 1997;273(6):F1023-F1029 - PMID: 29586122 - DOI: 10.1152/ajprenal.1997.273.6.F1023

  • Alper SL, Stuart-Tilley AK, Biemesderfer D, Shmukler BE, Brown D Immunolocalization of AE2 anion exchanger in rat kidney. Am J Physiol Renal Physiol. 1997;273(4):F601-F614 - PMID: 29587100 - DOI: 10.1152/ajprenal.1997.273.4.F601

  • Breton S, Smith PJS, Brown D Presence de cellules acidifiantes dans l'epididyme et le canal deferent: implication de la pompe a protons. Medecine/Sciences. 1997;13:57-61

  • Brown D, Smith PJ, Breton S Role of V-ATPase-rich cells in acidification of the male reproductive tract. J Exp Biol. 1997;200(Pt 2):257-62 - PMID: 9050233

  • Brown D, Breton S Mitochondria-rich, proton-secreting epithelial cells. J Exp Biol. 1996;199(Pt 11):2345-58 - PMID: 9114500

  • Breton S, Smith PJ, Lui B, Brown D Acidification of the male reproductive tract by a proton pumping (H+)-ATPase. Nature Med. 1996;2(4):470-2 - PMID: 8597961 - DOI: 10.1038/nm0496-470

  • Breton S, Alper SL, Gluck SL, Sly WS, Barker JE, Brown D Depletion of intercalated cells from collecting ducts of carbonic anhydrase II-deficient (CAR2 null) mice. Am J Physiol. 1995;269(6 Pt 2):F761-74 - PMID: 8594870 - DOI: 10.1152/ajprenal.1995.269.6.F761