Lin, Charles, PhD
I am an Associate Professor at Harvard Medical School and lead the Advanced Microscopy Group at the Center for Systems Biology and at the Wellman Center for Photomedicine, Massachusetts General Hospital. Here, my laboratory and I are developing cutting-edge optical imaging techniques for in vivo cell tracking and molecular imaging studies. Our primary research focus is the development of minimally invasive optical techniques for in vivo imaging of stem cells and hematologic malignancies. There are several custom-built confocal and two-photon hybrid microscopes within my laboratory, each tailored for a specific live animal imaging application. Several of these systems have an additional "treatment" beam that can be used to localize light delivery to precise locations in tissue. These systems have unique open architectures to allow modification and rapid adaptation to new technology. My laboratory has also developed an in vivo flow cytometer for real-time detection and quantification of fluorescent cells in the circulation, eliminating the need for drawing blood samples. At present, we are actively engaged in several multidisciplinary collaborative studies with experts across the fields of stem cell biology, immunology, and cancer biology.
Selected Publications (from total of 125)
Image-guided transplantation of single cells in the bone marrow of live animals.
mRNA-mediated glycoengineering ameliorates deficient homing of human stem cell-derived hematopoietic progenitors.
Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration.
Diffuse fluorescence fiber probe for in vivo detection of circulating cells.
Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging.
J Biophotonics. 2017;:ePub - PMID: 28164461
Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells.
Cell. 2016;167(5):1310-1322.e17 - PMID: 27863245
Self-renewal of a purified Tie2+ hematopoietic stem cell population relies on mitochondrial clearance.
Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators.
Tetrandrine identified in a small molecule screen to activate mesenchymal stem cells for enhanced immunomodulation.
Intravital multiphoton photoconversion with a cell membrane dye.
J Biophotonics. 2016;10(2):206-210 - PMID: 27433967
Corneal Crosslinking With Rose Bengal and Green Light: Efficacy and Safety Evaluation.
Cornea. 2016;35(9):1234-41 - PMID: 27362877
Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells.
Defining Clonal Color in Fluorescent
Distinct bone marrow blood vessels differentially regulate haematopoiesis.
Nature. 2016;532(7599):323-328 - PMID: 27074509
Macrophage IκB Kinase α Deficiency Suppresses Akt Phosphorylation, Reduces Cell Survival, and Decreases Early Atherosclerosis.