Lin, Charles, PhD
I am an Associate Professor at Harvard Medical School and lead the Advanced Microscopy Group at the Center for Systems Biology and at the Wellman Center for Photomedicine, Massachusetts General Hospital. Here, my laboratory and I are developing cutting-edge optical imaging techniques for in vivo cell tracking and molecular imaging studies. Our primary research focus is the development of minimally invasive optical techniques for in vivo imaging of stem cells and hematologic malignancies. There are several custom-built confocal and two-photon hybrid microscopes within my laboratory, each tailored for a specific live animal imaging application. Several of these systems have an additional "treatment" beam that can be used to localize light delivery to precise locations in tissue. These systems have unique open architectures to allow modification and rapid adaptation to new technology. My laboratory has also developed an in vivo flow cytometer for real-time detection and quantification of fluorescent cells in the circulation, eliminating the need for drawing blood samples. At present, we are actively engaged in several multidisciplinary collaborative studies with experts across the fields of stem cell biology, immunology, and cancer biology.
Selected Publications (from total of 132)
Fiber-based tunable repetition rate source for deep tissue two-photon fluorescence microscopy.
In Vivo 3D Histomorphometry Quantifies Bone Apposition and Skeletal Progenitor Cell Differentiation.
Fluorescence detection, enumeration and characterization of single circulating cells in vivo: technology, applications and future prospects.
Fluorescence detection, enumeration and characterization of single circulating cells in vivo technology, applications and future prospects.
Staged development of long lived TCRαβ Th17 resident memory T cell population to Candida albicans after skin infection.
Hormonal regulation of osteocyte perilacunar and canalicular remodeling in the Hyp mouse model of XLH.
Intravital imaging of osteocytes in mouse calvaria using third harmonic generation microscopy.
Image-guided transplantation of single cells in the bone marrow of live animals.
mRNA-mediated glycoengineering ameliorates deficient homing of human stem cell-derived hematopoietic progenitors.
Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration.
Diffuse fluorescence fiber probe for in vivo detection of circulating cells.
Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging.
Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells.
Self-renewal of a purified Tie2+ hematopoietic stem cell population relies on mitochondrial clearance.
Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators.