Lin, Charles, PhD
I am an Associate Professor at Harvard Medical School and lead the Advanced Microscopy Group at the Center for Systems Biology and at the Wellman Center for Photomedicine, Massachusetts General Hospital. Here, my laboratory and I are developing cutting-edge optical imaging techniques for in vivo cell tracking and molecular imaging studies. Our primary research focus is the development of minimally invasive optical techniques for in vivo imaging of stem cells and hematologic malignancies. There are several custom-built confocal and two-photon hybrid microscopes within my laboratory, each tailored for a specific live animal imaging application. Several of these systems have an additional "treatment" beam that can be used to localize light delivery to precise locations in tissue. These systems have unique open architectures to allow modification and rapid adaptation to new technology. My laboratory has also developed an in vivo flow cytometer for real-time detection and quantification of fluorescent cells in the circulation, eliminating the need for drawing blood samples. At present, we are actively engaged in several multidisciplinary collaborative studies with experts across the fields of stem cell biology, immunology, and cancer biology.
Selected Publications (from total of 90)
Direct measurement of local oxygen concentration in the bone marrow of live animals.
A new image of the hematopoietic stem cell vascular niche.
Cell Stem Cell. 2013;13(5):514-6 - PMID: 24209758
Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy.
Stress-induced hematopoietic failure in the absence of immediate early response gene X-1.
mRNA-engineered mesenchymal stem cells for targeted delivery of interleukin-10 to sites of inflammation.
Tracking single cells in live animals using a photoconvertible near-infrared cell membrane label.
Improved diffuse fluorescence flow cytometer prototype for high sensitivity detection of rare circulating cells in vivo.
In Vivo RNAi Screening Identifies a Leukemia-Specific Dependence on Integrin Beta 3 Signaling.
Soluble Guanylate Cyclase α1-Deficient Mice: A Novel Murine Model for Primary Open Angle Glaucoma.
In vivo tracking of hematopoietic cells in the retina of chimeric mice with a scanning laser ophthalmoscope
Advances in Single-cell Tracking of Mesenchymal Stem Cells (MSCs) During Musculoskeletal Regeneration.
High-Resolution Optical Mapping of Inflammatory Macrophages Following Endovascular Arterial Injury.
Inflammation Modulates Murine Venous Thrombosis Resolution In Vivo: Assessment by Multimodal Fluorescence Molecular Imaging.
Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy.
Tracking Mesenchymal Stem Cells with Iron Oxide Nanoparticle Loaded Poly(lactide-co-glycolide) Microparticles.