PI Bio

Lin, Charles, PhD

Charles_lin
Phone: 617-643-3531
Email:

Research

I am an Associate Professor at Harvard Medical School and lead the Advanced Microscopy Group at the Center for Systems Biology and at the Wellman Center for Photomedicine, Massachusetts General Hospital. Here, my laboratory and I are developing cutting-edge optical imaging techniques for in vivo cell tracking and molecular imaging studies. Our primary research focus is the development of minimally invasive optical techniques for in vivo imaging of stem cells and hematologic malignancies. There are several custom-built confocal and two-photon hybrid microscopes within my laboratory, each tailored for a specific live animal imaging application. Several of these systems have an additional "treatment" beam that can be used to localize light delivery to precise locations in tissue. These systems have unique open architectures to allow modification and rapid adaptation to new technology. My laboratory has also developed an in vivo flow cytometer for real-time detection and quantification of fluorescent cells in the circulation, eliminating the need for drawing blood samples. At present, we are actively engaged in several multidisciplinary collaborative studies with experts across the fields of stem cell biology, immunology, and cancer biology.

Selected Publications (from total of 90)

Spencer JA, Ferraro F, Roussakis E, Klein A, Wu J, Runnels JM, Zaher W, Mortensen LJ, Alt C, Turcotte R, Yusuf R, Côté D, Vinogradov SA, Scadden DT, Lin CP
Direct measurement of local oxygen concentration in the bone marrow of live animals.
Nature. 2014;508(7495):269-73 - PMID: 24590072 - PMCID: PMC3984353
Silberstein LE, Lin CP
A new image of the hematopoietic stem cell vascular niche.
Cell Stem Cell. 2013;13(5):514-6 - PMID: 24209758
Mortensen LJ, Levy O, Phillips JP, Stratton T, Triana B, Ruiz JP, Gu F, Karp JM, Lin CP
Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy.
PLoS ONE. 2013;8(10):e78145 - PMID: 24205131 - PMCID: PMC3812161
Ramsey H, Zhang Q, Brown DE, Steensma DP, Lin CP, Wu MX
Stress-induced hematopoietic failure in the absence of immediate early response gene X-1.
Haematologica. 2013;99(2):282-91 - PMID: 24056813 - PMCID: PMC3912958
Levy O, Zhao W, Mortensen LJ, Leblanc S, Tsang K, Fu M, Phillips JA, Sagar V, Anandakumaran P, Ngai J, Cui CH, Eimon P, Angel M, Lin CP, Yanik MF, Karp JM
mRNA-engineered mesenchymal stem cells for targeted delivery of interleukin-10 to sites of inflammation.
Blood. 2013;122(14):e23-e32 - PMID: 23980067 - PMCID: PMC3790516
Carlson AL, Fujisaki J, Wu J, Runnels JM, Turcotte R, Spencer JA, Celso CL, Scadden DT, Strom TB, Lin CP
Tracking single cells in live animals using a photoconvertible near-infrared cell membrane label.
PLoS ONE. 2013;8(8):e69257 - PMID: 23990881 - PMCID: PMC3753322
Pestana N, Mortensen LJ, Runnels JP, Vickers D, Murthy SK, Lin CP, Niedre M
Improved diffuse fluorescence flow cytometer prototype for high sensitivity detection of rare circulating cells in vivo.
J Biomed Opt. 2013;18(7):77002 - PMID: 23831714 - PMCID: PMC3702234
Miller PG, Al-Shahrour F, Hartwell KA, Chu LP, Järås M, Puram RV, Puissant A, Callahan KP, Ashton J, McConkey ME, Poveromo LP, Cowley GS, Kharas MG, Labelle M, Shterental S, Fujisaki J, Silberstein L, Alexe G, Al-Hajj MA, Shelton CA, Armstrong SA, Root DE, Scadden DT, Hynes RO, Mukherjee S, Stegmaier K, Jordan CT, Ebert BL
In Vivo RNAi Screening Identifies a Leukemia-Specific Dependence on Integrin Beta 3 Signaling.
Cancer Cell. 2013;24(1):45-58 - PMID: 23770013 - PMCID: PMC3746037
Buys ES, Ko YC, Alt C, Hayton SR, Jones A, Tainsh LT, Ren R, Giani A, Clerté M, Abernathy E, Tainsh RE, Oh DJ, Malhotra R, Arora P, de Waard N, Yu B, Turcotte R, Nathan D, Scherrer-Crosbie M, Loomis SJ, Kang JH, Lin CP, Gong H, Rhee DJ, Brouckaert P, Wiggs JL, Gregory MS, Pasquale LR, Bloch KD, Ksander BR
Soluble Guanylate Cyclase α1-Deficient Mice: A Novel Murine Model for Primary Open Angle Glaucoma.
PLoS ONE. 2013;8(3):e60156 - PMID: 23527308 - PMCID: PMC3603933
Alt C, Runnels J, Teo G, Lin C
In vivo tracking of hematopoietic cells in the retina of chimeric mice with a scanning laser ophthalmoscope
Intravital. 2012;1:132-140
Phillips JA, Mortensen LJ, Ruiz JP, Sridharan R, Kumar S, Torres M, Sharma P, Lin CP, Karp JM, Hauschka PV
Advances in Single-cell Tracking of Mesenchymal Stem Cells (MSCs) During Musculoskeletal Regeneration.
Orthop J Harv Med Sch. 2012;14:22-28 - PMID: 24244929 - PMCID: PMC3824625
Saxena A, Kessinger CW, Thompson B, McCarthy JR, Iwamoto Y, Lin CP, Jaffer FA
High-Resolution Optical Mapping of Inflammatory Macrophages Following Endovascular Arterial Injury.
Mol Imaging Biol. 2012;15(3):282-9 - PMID: 23090852 - PMCID: PMC3650124
Ripplinger CM, Kessinger CW, Li C, Kim JW, McCarthy JR, Weissleder R, Henke PK, Lin CP, Jaffer FA
Inflammation Modulates Murine Venous Thrombosis Resolution In Vivo: Assessment by Multimodal Fluorescence Molecular Imaging.
Arterioscler Thromb Vasc Biol. 2012;32(11):2616-24 - PMID: 22995524 - PMCID: PMC3516622
Wang K, Liu TM, Wu J, Horton NG, Lin CP, Xu C
Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy.
Biomed Opt Express. 2012;3(9):1972-7 - PMID: 23024893 - PMCID: PMC3447541
Xu C, Miranda-Nieves D, Ankrum JA, Matthiesen ME, Phillips J, Roes I, Wojtkiewicz GR, Juneja V, Kultima JR, Zhao W, Vemula PK, Lin CP, Nahrendorf M, Karp JM
Tracking Mesenchymal Stem Cells with Iron Oxide Nanoparticle Loaded Poly(lactide-co-glycolide) Microparticles.
Nano Lett. 2012;12(8):4131-9 - PMID: 22769232 - PMCID: PMC3552518
15

Charles Lin

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