Lin, Charles, PhD
I am an Associate Professor at Harvard Medical School and lead the Advanced Microscopy Group at the Center for Systems Biology and at the Wellman Center for Photomedicine, Massachusetts General Hospital. Here, my laboratory and I are developing cutting-edge optical imaging techniques for in vivo cell tracking and molecular imaging studies. Our primary research focus is the development of minimally invasive optical techniques for in vivo imaging of stem cells and hematologic malignancies. There are several custom-built confocal and two-photon hybrid microscopes within my laboratory, each tailored for a specific live animal imaging application. Several of these systems have an additional "treatment" beam that can be used to localize light delivery to precise locations in tissue. These systems have unique open architectures to allow modification and rapid adaptation to new technology. My laboratory has also developed an in vivo flow cytometer for real-time detection and quantification of fluorescent cells in the circulation, eliminating the need for drawing blood samples. At present, we are actively engaged in several multidisciplinary collaborative studies with experts across the fields of stem cell biology, immunology, and cancer biology.
Selected Publications (from total of 110)
Continuous volumetric imaging via an optical phase-locked ultrasound lens.
Nat Methods. 2015;12(8):759-762 - PMID: 26167641
Rapid Functional Decline of Activated and Memory Graft-versus-Host-Reactive T Cells Encountering Host Antigens in the Absence of Inflammation.
Analyzing Structure and Function of Vascularization in Engineered Bone Tissue by Video-rate Intravital Microscopy and 3D Image Processing.
Tissue Eng Part C Methods. 2015;:ePub - PMID: 25962617
A Small-Molecule Screen for Enhanced Homing of Systemically Infused Cells.
Statins Improve the Resolution of Established Murine Venous Thrombosis: Reductions in Thrombus Burden and Vein Wall Scarring.
Femtosecond laser bone ablation with a high repetition rate fiber laser source.
An authentic imaging probe to track cell fate from beginning to end.
Nat Commun. 2014;5:5216 - PMID: 25323442
Intravital Imaging of Mesenchymal Stem Cell Trafficking and Association with Platelets and Neutrophils.
Characterization of multiphoton microscopy in the bone marrow following intravital laser osteotomy.
Inhibiting stromal cell heparan sulfate synthesis improves stem cell mobilization and enables engraftment without cytotoxic conditioning.
Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis.
In vivo imaging of microglia turnover in the mouse retina after ionizing radiation and dexamethasone treatment.
Invest Ophthalmol Vis Sci. 2014;55(8):5314-5319 - PMID: 25082884
Intravital imaging of hematopoietic stem cells in the mouse skull.
Methods Mol Biol. 2014;1185:247-65 - PMID: 25062634
ABCB5 is a limbal stem cell gene required for corneal development and repair.
Silencing of CCR2 in myocarditis.